Bos taurus variety ‘HO840003150607238’ and methods of use thereof

Patent metadata

Field	Value
Publication number	US-10982187-B2
Application number	US-201916365306-A
Country	US
Kind code	B2
Filing date	Mar 26, 2019
Priority date	Mar 26, 2019
Publication date	Apr 20, 2021
Grant date	Apr 20, 2021

Abstract

The disclosure relates to Bovine germplasm of Bos taurus variety HO840M003150607238. Included in the present disclosure are cells comprising the genome of Bovine variety HO840M003150607238 characterized by the presence of homozygous loci and spermatozoa obtained from said cells. Also provided by the present disclosure are tissue cultures of cells, animals obtained from said cells, and parts thereof, including F1 spermatozoa. The disclosure further provides for methods of breeding, selecting, and using the germplasm to improve existing commercial cattle herds generated from in vitro fertilization methods and progeny cattle obtained from in vitro fertilization and implantation and artificial insemination methods.

First claim

The invention claimed is:

1. A plurality of Bos taurus cells comprising HO840003150607238 germplasm, wherein a representative sample of HO840003150607238 germplasm has been deposited under ATCC Accession No. PTA-125702.

2. A frozen vial, a cell culture, a tissue, a zygote, or an embryo comprising the plurality of Bos taurus cells according to claim 1 .

3. A plurality of Bos taurus gamete cells comprising HO840003150607238 germplasm, wherein a representative sample of HO840003150607238 germplasm has been deposited under ATCC Accession No. PTA-125702.

4. Fresh semen comprising the plurality of Bos taurus gamete cells according to claim 3 , wherein said gamete cells are sperm cells.

5. A cryopreserved bovine inseminate comprising the plurality of Bos taurus gamete cells according to claim 3 .

6. An F1 Bos taurus animal, or part thereof, wherein said F1 Bos taurus animal is an F1 progeny animal of a Bos taurus animal comprising HO840003150607238 germplasm, wherein a representative sample of HO840003150607238 germplasm has been deposited under ATCC Accession No. PTA-125702.

7. The F1 Bos taurus animal, or part thereof, according to claim 6 , wherein said F1 Bos taurus animal is prepared by artificial conception.

8. The F1 Bos taurus animal, or part thereof, according to claim 7 , wherein said artificial conception is in vitro fertilization and implantation, Artificial Insemination (AI), or in vivo insemination and embryo transfer (ET).

9. The F1 Bos taurus animal, or part thereof, according to claim 6 , wherein said part thereof is an isolated oocyte or a plurality of sperm.

10. An inseminate composition comprising cryoprotectant and semen comprising Bos taurus HO840003150607238 gamete cells according to claim 3 , wherein the gamete cells are sperm cells.

11. The F1 Bos taurus animal, or part thereof, according to claim 6 , said part comprising an F1 Bos taurus inseminate comprising at least half of a haploid genome of Bos taurus HO840003150607238 germplasm.

12. A bull comprising the plurality of Bos taurus cells according to claim 1 .

13. The plurality of Bos taurus gamete cells according to claim 3 , wherein the gamete cells are sperm cells.

14. Semen comprising the plurality of Bos taurus sperm cells according to claim 13 .

15. Semen according to claim 14 , wherein the semen is an inseminate.

16. Semen according to claim 15 , wherein said inseminate is contained in a straw.

17. Semen comprising the plurality of sperm according to claim 9 .

18. Semen according to claim 17 , wherein the semen is an inseminate.

19. Semen according to claim 18 , wherein said inseminate is contained in a straw.

20. Semen according to claim 19 , wherein the inseminate is cryopreserved.

21. The F1 Bos taurus animal, or part thereof, according to claim 6 , comprising a gene edited genome.

22. The F1 Bos taurus animal, or part thereof, according to claim 21 , wherein said gene edited genome is the gene edited genome of Bos taurus inseminate comprising at least half of a haploid genome of Bos taurus HO840003150607238 germplasm.

Assignees

• Abs Global Inc
• Genus Plc

Inventors

• Funk Devan Charles
• Dattilo Katrina

Classifications

• C12N5/0611 Primary

  Primordial germ cells, e.g. embryonic germ cells [EG] · CPC title

• A01K67/027

  New or modified breeds of vertebrates · CPC title

• C12N5/0609

  Oocytes, oogonia (fertilised oocytes C12N5/0604) · CPC title

• C12N15/8771 Primary

  Bovine embryos · CPC title

• C12N5/061

  Sperm cells, spermatogonia · CPC title