Amylases, nucleic acids encoding them and methods for making and using them
US-9249400-B2 · Feb 2, 2016 · US
US10980249B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10980249-B2 |
| Application number | US-201515317697-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 29, 2015 |
| Priority date | Jun 27, 2014 |
| Publication date | Apr 20, 2021 |
| Grant date | Apr 20, 2021 |
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The invention relates to the use of at least one bacterial phytase in combination with one or more protease(s) in animal feed for improving weight gain and/or Feed Conversion Ratio (FCR), wherein the phytase is administered in one or more of the following amounts (dosage ranges): 1,000 FYT/kg feed, 2,000 FYT/kg feed 3,000 FYT/kg feed and wherein the protease is administered in one of the following amounts (dosage ranges): 10,000 units/kg feed, 11,000, 12,000, 13,000, 14,000, 15,000, 16,000, 17,000, 18,000, 19,000, 20,000 units/kg feed.
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The invention claimed is: 1. A method for improving Feed Conversion Ratio of a farm animal, comprising applying to the animal a feed comprising at least one protease and at least one phytase wherein: a. the phytase is administered in an amount that the specific activity in the feed is 3000 FYT/kg feed and b. the protease is administered at a dosage of between 10000 units/kg feed and 30000 units/kg feed. 2. The method of claim 1 , the protease is administered at a dosage selected from the group consisting of 10000, 11000, 12000, 13000, 14000, 15000, 16000, 17000, 18000, 19000, and 20000 units/kg feed. 3. The method of claim 1 , wherein the protease is administered at a dosage of 15000 units/kg feed. 4. The method of claim 1 , wherein the phytase is classified as belonging to the EC 3.1.3.26 group. 5. The method of claim 1 , wherein the phytase is derived from the family Enterobacteriaceae. 6. The method of claim 1 , wherein the protease is an acid stable serine protease obtained or obtainable from the class Actinobacteria. 7. The method of claim 1 , wherein the protease comprises the amino acid sequence of any of SEQ ID NOs: 27-48. 8. The method of claim 1 , wherein the farm animal is selected from the group consisting of pigs, swine, poultry, turkeys, ducks, quail, guinea fowl, geese, pigeons and chicken. 9. A method for improving Feed Conversion Ratio of a farm animal, comprising applying to the animal a feed comprising at least one protease and at least one phytase wherein: a. the phytase is administered in an amount that the specific activity in the feed is 3000 FYT/kg feed and b. the protease is administered at a dosage of 15000 units/kg feed. 10. The method of claim 9 , wherein the phytase is classified as belonging to the EC 3.1.3.26 group. 11. The method of claim 9 , wherein the phytase is derived from the family Enterobacteriaceae. 12. The method of claim 9 , wherein the protease is an acid stable serine protease obtained or obtainable from the class Actinobacteria. 13. The method of claim 9 , wherein the protease comprises the amino acid sequence of any of SEQ ID NOs: 27-48. 14. The method of claim 9 , wherein the farm animal is selected from the group consisting of pigs, swine, poultry, turkeys, ducks, quail, guinea fowl, geese, pigeons and chicken.
4-Phytase (3.1.3.26), i.e. 6-phytase · CPC title
Enzymes · CPC title
Hydrolases acting on peptide bonds, i.e. peptidases (3.4) · CPC title
acting on peptide bonds (3.4) · CPC title
Pretreatment of feeding-stuffs with enzymes · CPC title
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