Magnetic enrichment of magnetically marked analytes

Patent metadata

Field	Value
Publication number	US-10976227-B2
Application number	US-201515552248-A
Country	US
Kind code	B2
Filing date	Nov 16, 2015
Priority date	Feb 19, 2015
Publication date	Apr 13, 2021
Grant date	Apr 13, 2021

Abstract

The present subject matter describes system (100) for magnetic enrichment of magnetically marked analytes. The system has capture chip (102) comprising a sample chamber (104) for holding sample having magnetically marked analytes, and a recovery chamber (106) connected to the sample chamber by a channel (202). The volume of recovery chamber is smaller than volume of sample chamber (104). The system has magnetic arrangement (108) comprising a set of magnets (110) in which each two adjacent magnets have opposite polarities facing sample chamber. Set of magnets has dimensions that at least conform to coverage area of sample chamber. The magnetic arrangement also has at least one recovery magnet (112) having dimensions conforming to coverage area of recovery chamber and on alignment, the at least one recovery magnet (112) is at a distance farthest away from the recovery chamber. The system has a linear positioner (114) for moving the magnetic arrangement.

First claim

We claim:

1. A system ( 100 ) for magnetic enrichment of magnetically marked analytes, wherein the system ( 100 ) comprises:
a capture chip ( 102 ) comprising:
a sample chamber ( 104 ) for holding a sample having the magnetically marked analytes; and
a recovery chamber ( 106 ) connected to the sample chamber ( 104 ) by a channel ( 202 ), wherein volume of the recovery chamber ( 106 ) is smaller than volume of the sample chamber ( 104 ) wherein the recovery chamber ( 106 ) and the channel ( 202 ) are at a corner of the sample chamber ( 104 ) and are aligned along a diagonal of the sample chamber ( 104 );

a magnetic arrangement ( 108 ) comprising:
a set of magnets ( 110 ) for providing an external magnetic field to the magnetically marked analytes in the sample chamber ( 104 ), wherein each two adjacent magnets of the set of magnets ( 110 ) have opposite polarities facing the sample chamber ( 104 ), and wherein the set of magnets ( 110 ) has dimensions that at least conforms to an area of the sample chamber ( 104 ) in which the sample is held; and

at least one recovery magnet ( 112 ) at a corner of the set of magnets ( 110 ), wherein the at least one recovery magnet ( 112 ) has dimensions that at least conforms to an area of the recovery chamber ( 106 ) in which the magnetically marked analytes are dragged, and wherein the magnetic arrangement ( 108 ) is aligned with the capture chip ( 102 ) such that the at least one recovery magnet ( 112 ) is at a distance farthest away from the recovery chamber ( 106 );
a linear positioner ( 114 ) coupled to the magnetic arrangement ( 108 ); and
a stepper motor ( 124 ) coupled to the linear positioner ( 114 ) to move the linear positioner ( 114 ) for moving the magnetic arrangement ( 108 ) with respect to the capture chip ( 102 ) along an axis passing through the recovery chamber ( 106 ) and the at least one recovery magnet ( 112 ),
wherein the magnetic arrangement ( 108 ) is positioned with respect to the capture chip ( 102 ) such that the at least one recovery magnet ( 112 ) is located at a corner of the sample chamber ( 104 ) diagonally opposite to the corner thereof at which the recovery chamber ( 106 ) is located, and wherein the magnetic arrangement ( 108 ) is moved by the linear positioner ( 114 ) along the diagonal of the sample chamber ( 104 ).

2. The system ( 100 ) as claimed in claim 1 , wherein the coverage area of the sample chamber ( 104 ) has a square shape.

3. The system ( 100 ) as claimed in claim 2 , wherein the set of magnets ( 110 ) of the magnetic arrangement ( 108 ) are arranged in an N ×N grid that conforms to the shape of the coverage area of the sample chamber ( 104 ).

Assignees

• Indian Institute Tech Delhi

Inventors

• Elangovan Ravikrishnan
• Perumal Vivekanandan
• Gupta Shalini
• Singh Saurabh
• Upadhyay Mohita

Classifications

• C12M47/04 Primary

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title

• B03C1/01 Primary

  by addition of magnetic adjuvants · CPC title

• B01L2400/06

  Valves, specific forms thereof · CPC title

• B03C1/288

  disposed at the outer circumference of a recipient · CPC title

• B03C2201/22

  characterised by the magnetic field, e.g. its shape or generation · CPC title