Clostridium sp. strain producing hexanoic acid in high yield and method for producing hexanoic acid using the same

Patent metadata

Field	Value
Publication number	US-10975448-B2
Application number	US-201916253581-A
Country	US
Kind code	B2
Filing date	Jan 22, 2019
Priority date	Jan 23, 2018
Publication date	Apr 13, 2021
Grant date	Apr 13, 2021

Abstract

The present disclosure relates to a Clostridium sp. JS66 strain producing metabolites having 4 to 6 carbon atoms in a high yield. The strain produces metabolites having 6 carbon atoms in a significantly high yield while reducing the production of acetic acid and ethanol as by-products.

First claim

What is claimed is:

1. A medium composition comprising:
a Clostridium sp. JS66 strain of accession No. KCTC13355BP producing metabolites having 4 to 6 carbon atoms; and
an effective amount of a substrate for production of said metabolites;
wherein said substrate comprises:
carbon monoxide and carbon dioxide;
glucose; and
at least one sugar-based carbon source of fructose, galactose, xylose, arabinose, mannose, cellobiose, and sucrose; and

wherein said metabolites comprise:
hexanoic acid; and
at least one of butyric acid, butanol and hexanol.

2. The medium composition according to claim 1 ,
wherein the metabolites further comprise at least one of butyric acid and hexanol.

3. The medium composition according to claim 1 ,
wherein the Clostridium sp. strain produces the metabolites using the substrate, wherein said substrate comprises:
glucose and at least one sugar-based carbon source of galactose, xylose, arabinose, mannose, cellobiose, and sucrose.

4. The medium composition according to claim 1 ,
wherein the Clostridium sp. strain produces the metabolites using a mixed gas further comprising hydrogen and of said carbon monoxide and carbon dioxide.

5. The medium composition according to claim 1 ,
wherein the optimal culture temperature is 25° C. to 30° C.

6. A method for producing metabolites having 4 to 6 carbon atoms, comprising supplying Clostridium sp. JS66 strain, thereby producing said metabolites of accession No. KCTC13355BP to a substrate comprising a carbon source and culturing the strain.

7. The method for production according to claim 6 ,
wherein the culture is carried out at 25° C. to 30° C.

8. The method for production according to claim 6 ,
wherein the culture is carried out at pH 5 to pH 8.

9. The method for production according to claim 6 ,
wherein the culture is carried out under anaerobic conditions.

10. The method for production according to claim 6 ,
wherein the substrate comprises at least one of glucose, fructose, galactose, xylose, arabinose, mannose, cellobiose, and sucrose.

11. The method for production according to claim 6 ,
wherein the substrate comprises at least one of carbon monoxide and carbon dioxide.

12. The method for production according to claim 11 ,
wherein the substrate further comprises hydrogen.

13. The method for production according to claim 11 ,
wherein the culture is carried out at a total gas pressure of 3 bar or less.

14. The method for production according to claim 6 ,
wherein the culture is carried out in a medium containing yeast extract.

15. The method for production according to claim 14 ,
wherein the medium contains 0.1 g/L to 10 g/L of yeast extract.

16. A method for fixing carbon monoxide and carbon dioxide, comprising supplying a mixed gas comprising carbon monoxide and carbon dioxide to a culture of Clostridium sp. JS66 strain of accession No. KCTC13355BP and culturing said strain, thereby fixing said carbon monoxide and carbon dioxide.

17. The method for fixing carbon monoxide and carbon dioxide according to claim 16 , further comprising producing metabolites having 4 to 6 carbon atoms from carbon monoxide and carbon dioxide.

Assignees

• Korea Inst Sci & Tech

Inventors

• Um Youngsoon
• Kim Joongsuk
• Lee Sun-Mi
• Gong Gyeongtaek

Classifications

• C12R2001/145

  Clostridium · CPC title

• C12P7/52 Primary

  Propionic acid; Butyric acids · CPC title

• C12N1/205 Primary

  Bacterial isolates · CPC title

• C12P7/04

  acyclic · CPC title

• C12P7/40

  containing a carboxyl group {including Peroxycarboxylic acids} · CPC title