CRISPR hybrid DNA/RNA polynucleotides and methods of use
US-9868962-B2 · Jan 16, 2018 · US
US10975351B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10975351-B2 |
| Application number | US-201916443418-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 17, 2019 |
| Priority date | Jun 17, 2019 |
| Publication date | Apr 13, 2021 |
| Grant date | Apr 13, 2021 |
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
What the patent document calls the invention.
A short plain-language summary of the technical disclosure.
Who owns or filed the patent and who is credited as inventor.
Filing, priority, publication, and grant dates set the timeline.
The legal scope of protection — read this for what is actually claimed.
Technology tags used to group this patent with similar filings.
Prior art links and similar publications in this corpus.
Official abstract text for this publication.
The disclosure relates to Bovine germplasm of Bos taurus variety JE840003146074527. Included in the present disclosure are cells comprising the Bovine variety JE840003146074527. Also provided by the present disclosure are tissue cultures of cells, animals obtained from said cells, and parts thereof, including F1 spermatozoa. The disclosure further provides for methods of breeding, selecting, and using the germplasm to improve existing commercial cattle herds generated from in vitro fertilization methods and progeny cattle obtained from in vitro fertilization and implantation and artificial insemination methods.
Opening claim text (preview).
What is claimed is: 1. A Bos taurus cell comprising JE840003146074527 germplasm, a representative sample of JE840003146074527 germplasm having been deposited under ATCC accession number PTA-126146. 2. A frozen vial, a cell culture, a tissue, a zygote, or an embryo comprising a plurality of the Bos taurus cell according to claim 1 . 3. A cow or heifer comprising a plurality of the Bos taurus cell according to claim 1 . 4. The Bos taurus cell according to claim 1 , wherein the cell is an oocyte or an ova matured therefrom. 5. The Bos taurus oocyte or ova according to claim 4 , wherein the oocyte or an ova matured therefrom is an isolated oocyte or an ova matured therefrom. 6. The Bos taurus oocyte or ova according to claim 5 , wherein the oocyte or an ova matured therefrom is isolated for in vitro fertilization. 7. A Bos taurus cell produced by somatic cell nuclear transfer of JE840003146074527 germplasm, a representative sample of JE840003146074527 germplasm having been deposited under ATCC accession number PTA-126146. 8. A frozen vial, a cell culture, a tissue, a zygote, or an embryo comprising a plurality of the Bos taurus cell according to claim 7 . 9. A cow or heifer comprising a plurality of the Bos taurus cell according to claim 7 . 10. The Bos taurus cell according to claim 7 , wherein the genome comprises at least one edited gene. 11. A Bos taurus cell from an F1 offspring of a Bos taurus animal comprising JE840003146074527 germplasm, a representative sample of JE840003146074527 germplasm having been deposited under ATCC accession number PTA-126146. 12. Meat comprising a plurality of the Bos taurus cell from an F1 offspring cell according to claim 11 . 13. A frozen vial, a cell culture, a tissue, or a zygote comprising a plurality of the Bos taurus cell according to claim 11 . 14. A bull comprising the Bos taurus cell from an F1 offspring according to claim 11 . 15. A cow or heifer comprising the Bos taurus cell from an F1 offspring according to claim 11 . 16. The Bos taurus cell from an F1 offspring according to claim 11 , wherein the offspring is conceived using in vitro fertilization. 17. The Bos taurus cell from an F1 offspring according to claim 11 , wherein the offspring is conceived by artificial insemination of a superovulated cow or heifer. 18. The Bos taurus cell from an F1 offspring according to claim 17 , wherein the offspring is gestated by a Bos taurus surrogate mother. 19. The Bos taurus cell from an F1 offspring according to claim 11 , wherein the offspring is conceived by in vitro fertilization of an ovum obtained from a superovulated cow or heifer. 20. The Bos taurus cell from an F1 offspring according to claim 19 , wherein the offspring is gestated by a Bos taurus surrogate mother. 21. The Bos taurus cell from an F1 offspring according to claim 11 , wherein the F1 offspring is a gene edited Bos taurus animal. 22. A Bos taurus cell from an F1 offspring according to claim 11 , wherein the cell is a haploid cell. 23. The Bos taurus haploid cell from an F1 offspring according to claim 22 , wherein the cell is a gamete. 24. The Bos taurus haploid cell from an F1 offspring according to claim 22 , wherein the cell is an ovum. 25. The Bos taurus haploid cell from an F1 offspring according to claim 22 , wherein the cell is a sperm. 26. Semen comprising a plurality of the Bos taurus sperm according to claim 25 . 27. Semen according to claim 26 , wherein the semen is contained in a straw. 28. Semen according to claim 27 , wherein the semen is cryopreserved. 29. The Bos taurus cell according to claim 22 , wherein the haploid cell comprises at least one edited gene. 30. A container of semen produced by the bull of claim 14 .
Carriers for immersion in cryogenic fluid for slow freezing or vitrification · CPC title
Germ cells (production of embryos, nuclear transfer A01K67/027) · CPC title
Sperm cells, spermatogonia · CPC title
New or modified breeds of vertebrates · CPC title
Animal zootechnically ameliorated · CPC title
Related publications grouped by family.
Answers are generated from the same data shown on this page.