Valuable product and method for obtaining a valuable material phase

Patent metadata

Field	Value
Publication number	US-10975244-B2
Application number	US-201716328195-A
Country	US
Kind code	B2
Filing date	Aug 17, 2017
Priority date	Aug 26, 2016
Publication date	Apr 13, 2021
Grant date	Apr 13, 2021

Abstract

The present invention relates to a valuable product in the form of a flavonoid-containing phenol mixture having intense red coloration and to a method for obtaining a valuable-substance phase, more particularly a red-colored phase, from a native substance mixture.

First claim

The invention claimed is:

1. A method for obtaining a valuable-substance phase comprising the following steps:
Step A: providing a native substance mixture composed of seeds and/or fruits from the cabbage family Brassicaceae, having comminuted as a proportion of hard, breakable shells or in shelled form, wherein the native substance mixture is rapeseeds composed of complete seeds or composed of partially deoiled seeds;
Step B: if the native substance mixture from step A) is not completely comminuted, further comminuting the native substance mixture from step A) to break up the shells;
Step C: dispersing and/or mixing the comminuted substance mixture from step A) or B) with water, with addition of up to a maximum of 8 parts water to one part comminuted substance mixture and with stirring of the water and the comminuted substance mixture to yield a flowable slurry or a dispersion;
Step D: adjusting the pH of the slurry from step C) into an alkaline range of pH>9.5;
Step E: adding a water-soluble organic solvent to the slurry from step D) following the adjustment of the pH of the slurry in step D); in such a way that an alcohol concentration of less than 30% by volume is reached in order to detach the shells from the endosperm of the seeds/fruits;
Step F: separating off a solid phase comprising the overwhelming proportion of any shells still present, wherein the separation is performed in the centrifugal field in a centrifuge;
Step G: shifting the pH of the solid phase-cleared slurry from step F) into the pH range of from pH=4.5 to pH=7.2; and
Step H: separating the shell-free slurry, the pH of which was shifted into the acidic range in step G), wherein the separation is performed in a centrifuge into multiple phases, wherein at least one of said phases is a sinapinic acid-containing phase;
Step I: adding laccase to the sinapinic acid-containing phase of step H),
wherein the phase separation is performed in step H) in one or two steps, into two valuable-substance phases, with at least one aqueous phase comprising an albumin content and a sinapinic acid content and a residual-oil content.

2. The method as claimed in claim 1 , characterized in that the native substance mixture is processed in the form of a recently produced intermediate product not more than 31 days after a preliminary stage.

3. The method as claimed in claim 1 , characterized in that the native substance mixture is processed in the form of a fresh intermediate product not more than 3 days after a preliminary stage.

4. The method as claimed in claim 1 , characterized in that the native substance mixture used in step A) is a cold-pressed material, which was pressed at a temperature of less than 70° C.

5. The method as claimed in claim 1 , characterized in that one or more of the separation steps in step F) or H) is/are carried out in a 3-phase decanter or in at least two steps in 2-phase decanters.

6. The method as claimed in claim 1 , characterized in that one or more of the separation steps in step F) or H) is/are carried out in a nozzle separator.

7. The method as claimed in claim 1 , wherein the water-soluble organic solvent is a linear aliphatic alcohol.

8. The method as claimed in claim 1 , wherein the content of the water-soluble organic solvent in the aqueous part of the slurry in step E) after the addition of the water-soluble organic solvent is 20% by volume or less.

9. The method as claimed in claim 1 , wherein all the method steps are performed at a temperature below 60° C.

10. The method as claimed in claim 1 , wherein all the method steps are performed at a temperature below 50° C.

11. A method for obtaining a valuable-substance phase comprising the following steps:
Step A: providing a native substance mixture composed of seeds and/or fruits from the cabbage family Brassicaceae, having comminuted as a proportion of hard, breakable shells or in shelled form, wherein the native substance mixture is rapeseeds composed of complete seeds or composed of partially deoiled seeds;
Step B: if the native substance mixture from step A) is not completely comminuted, further comminuting the native substance mixture from step A) to break up the shells;
Step C: dispersing and/or mixing the comminuted substance mixture from step A) or B) with water, with addition of up to a maximum of 8 parts water to one part comminuted substance mixture and with stirring of the water and the comminuted substance mixture to yield a flowable slurry or a dispersion;
Step D: adjusting the pH of the slurry from step C) into an alkaline range of pH>9.5;
Step E: adding a water-soluble organic solvent to the slurry from step D) following the adjustment of the pH of the slurry in step D); in such a way that an alcohol concentration of less than 30% by volume is reached in order to detach the shells from the endosperm of the seeds/fruits;
Step F: separating off a solid phase comprising the overwhelming proportion of any shells still present, wherein the separation is performed in the centrifugal field in a centrifuge;
Step G: shifting the pH of the solid phase-cleared slurry from step F) into the pH range of from pH=4.5 to pH=7.2;
Step H: separating the shell-free slurry, the pH of which was shifted into the acidic range in step G), wherein the separation is performed in a decanter into multiple phases, wherein at least one of said phases is a sinapinic acid-containing phase; and
Step I: adding laccase to the sinapinic acid-containing phase of step H),
wherein the phase separation is performed in step H) in one or two steps, into two valuable-substance phases, with at least one aqueous phase comprising an albumin content and a sinapinic acid content and a residual-oil content.

12. The method as claimed in claim 3 , wherein the native substance mixture is processed in the form of a fresh intermediate product less than 48 hours after the preliminary stage.

13. The method as claimed in claim 3 , wherein the native substance mixture is processed in the form of a fresh intermediate product less than 24 hours after the preliminary stage.

14. The method as claimed in claim 4 , wherein the cold pressed material is a cold pressed rape cake.

15. The method as claimed in claim 4 , wherein the cold-pressed material was pressed at a temperature of less than 60° C.

16. The method as claimed in claim 1 , wherein in step C), the dispersing and/or mixing of the comminuted substance mixture from step A) or B) with water is with the addition of up to a maximum of 6 parts water to one part comminuted substance mixture.

17. The method as claimed in claim 1 , wherein in step C), the dispersing and/or mixing of the comminuted substance mixture from step A) or B) with water is with the addition of up to a maximum of 5 parts water to one part comminuted substance mixture.

18. The method as claimed in claim 1 , wherein in step E), the water-soluble organic solvent is ethanol and is in water diluted form.

19. The method as claimed in claim 1 , wherein the content of the water-soluble organic solvent added to the slurry in step E) is 30 to 60% by volume.

Assignees

• Gea Mechanical Equipment Gmbh

Inventors

• Hruschka Steffen
• Boszulak Wladislawa
• Martel Daniel Michael

Classifications

• C12N9/0061 Primary

  Laccase (1.10.3.2) · CPC title

• C12N9/0004

  Oxidoreductases (1.) · CPC title

• A23L11/30

  Removing undesirable substances, e.g. bitter substances · CPC title

• A23K10/37

  from waste material (from hydrolysates of wood or straw A23K10/32; from molasses A23K10/33) · CPC title

• A23J1/14

  from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds · CPC title