Irradiated membrane for cell expansion

Patent metadata

Field	Value
Publication number	US-10974201-B2
Application number	US-200913120375-A
Country	US
Kind code	B2
Filing date	Sep 23, 2009
Priority date	Sep 25, 2008
Publication date	Apr 13, 2021
Grant date	Apr 13, 2021

Abstract

A membrane for cultivating adherent or suspension cells, in particular adherent cells. The membrane permits adhesion and proliferation of the cells due to the irradiation of the wet or dry membrane with gamma or beta rays or an electron beam in a dose of from 12.5 to 175 kGy in the presence of oxygen. The resulting membrane may be used without any pre-treatment with surface-modifying substances. A method for preparing such an irradiated membrane for cultivating adherent or suspension cells. Methods of using such a membrane for cultivating adherent or suspension cells.

First claim

The invention claimed is:

1. A cell culturing device comprising a) a membrane comprising i) a component selected from the group consisting of a polysulfone, polyethersulfone, a polyarylethersulfone, and combinations thereof, and ii) a polyvinylpyrrolidone, wherein the membrane has been irradiated with at least one of gamma-rays or beta-rays in a dose of from 70 to 175 kGy in the presence of oxygen at a concentration of 4 to 100 vol %, and b) adherent cells.

2. A device for the extracorporeal treatment of body fluids, the device comprising a) adherent cells and b) an irradiated membrane including at least one of a lumen surface and an outer surface, the membrane comprising i) a component selected from the group consisting of a polysulfone, polyethersulfone, a polyarylethersulfone, and combinations thereof, and ii) a polyvinylpyrrolidone,
wherein the adherent cells form a confluent layer on the lumen surface or outer surface of the membrane, and
wherein the membrane has been irradiated with at least one of gamma-rays or beta-rays in a dose of from 70 to 175 kGy in the presence of oxygen at a concentration of 4 to 100 vol %.

3. The cell culturing device of claim 1 , wherein the adherent cells are selected from the group consisting of stem cells, embryonic stem cells, adult stem cells, mesenchymal stem cells (MSC), hematopoietic stem cells, cord blood cells, neural stem cells, smooth muscle cells, skin cells, nerve cells, neuroglia cells, endothelial cells, epithelial cells, fibroblasts, hepatocytes, endothelial cells, muscle cells, and chondrocytes.

4. The cell culturing device of claim 1 , wherein the adherent cells are selected from the group consisting of mesenchymal stem cells (MSC), fibroblasts, epithelial cells, and hepatocytes.

5. The device of claim 2 , wherein the adherent cells are selected from the group consisting of stem cells, embryonic stem cells, adult stem cells, mesenchymal stem cells (MSC), hematopoietic stem cells, cord blood cells, neural stem cells, smooth muscle cells, skin cells, nerve cells, neuroglia cells, endothelial cells, epithelial cells, fibroblasts, hepatocytes, endothelial cells, muscle cells, and chondrocytes.

6. The device of claim 2 , wherein the adherent cells are selected from the group consisting of mesenchymal stem cells (MSC), fibroblasts, epithelial cells, and hepatocytes.

7. The cell culturing device of claim 1 , wherein the membrane further comprises a polyurethane.

8. The cell culturing device of claim 1 , wherein the membrane further comprises a polyamide.

9. The cell culturing device of claim 1 , wherein the membrane is a hollow fiber membrane.

10. The cell culturing device of claim 1 , wherein the membrane is a flat sheet membrane.

11. The cell culturing device of claim 1 , wherein the membrane has been irradiated in the presence of oxygen at a concentration of 4 to 30 vol %.

12. The cell culturing device of claim 1 , wherein the polyvinylpyrrolidone comprises a blend of at least two polyvinylpyrrolidone (PVP) homopolymers, wherein the first PVP homopolymer is a low molecular weight PVP comprising an average relative molecular weight of from about 10,000 g/mol to 100,000 g/mol and wherein the second PVP homopolymer is a high molecular weight PVP comprising an average relative molecular weight of from about 500,000 g/mol to 2,000,000 g/mol.

13. The cell culturing device of claim 9 , wherein the hollow fiber membrane comprises an interior of the hollow fiber membrane and an exterior of the hollow fiber membrane, and wherein the adherent cells are present on the interior of the hollow fiber membrane and the exterior of the hollow fiber membrane.

14. The device of claim 2 , wherein the membrane further comprises a polyurethane.

15. The device of claim 2 , wherein the membrane further comprises a polyamide.

16. The device of claim 2 , wherein the membrane is a hollow fiber membrane.

17. The device of claim 2 , wherein the membrane is a flat sheet membrane.

18. The device of claim 2 , wherein the membrane has been irradiated in the presence of oxygen at a concentration of 4 to 30 vol %.

19. The device of claim 2 , wherein the polyvinylpyrrolidone comprises a blend of at least two polyvinylpyrrolidone (PVP) homopolymers, wherein the first PVP homopolymer is a low molecular weight PVP comprising an average relative molecular weight of from about 10,000 g/mol to 100,000 g/mol and wherein the second PVP homopolymer is a high molecular weight PVP comprising an average relative molecular weight of from about 500,000 g/mol to 2,000,000 g/mol.

20. The device of claim 16 , wherein the hollow fiber membrane comprises an interior of the hollow fiber membrane and an exterior of the hollow fiber membrane, and wherein the adherent cells are present on the interior of the hollow fiber membrane and the exterior of the hollow fiber membrane.

Assignees

• Krause Bernd
• Neubauer Markus
• Loercher Joachim
• Gambro Lundia Ab

Inventors

• Krause Bernd
• Neubauer Markus
• Loercher Joachim

Classifications

• A61L2/022

  Filtration · CPC title

• B01D67/009 Primary

  with wave-energy, particle-radiation or plasma · CPC title

• C12M25/02

  Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00) · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• C12N2533/52

  Fibronectin; Laminin · CPC title