Patterned electrospun fibers for tissue engineering

Patent metadata

Field	Value
Publication number	US-10973957-B2
Application number	US-201816476874-A
Country	US
Kind code	B2
Filing date	Jan 10, 2018
Priority date	Jan 10, 2017
Publication date	Apr 13, 2021
Grant date	Apr 13, 2021

Abstract

A composition of matter for tissue engineering is disclosed. The composition comprises a plurality of electrospun albumin fibers, wherein an outer surface of the composition comprises a pattern of ridges or indentations, wherein the ridges or indentations are wider than the diameter of a single electrospun albumin fiber of the plurality of electrospun albumin fibers.

First claim

What is claimed:

1. A scaffold for tissue engineering comprising at least two layers, wherein each of said at least two layers are fabricated from a composition of matter which comprises a plurality of electrospun albumin fibers, wherein an outer surface of the composition comprises a pattern of ridges or indentations, wherein the width of an individual ridge or indentation of said pattern of ridges or indentations is wider than the diameter of a single electrospun albumin fiber of said plurality of electrospun albumin fibers, each layer comprising a pattern with distinct indentations or ridges.

2. The scaffold of claim 1 , wherein at least one of the layers is seeded with cells.

3. The scaffold of claim 2 , wherein said cells are selected from the group consisting of cardiomyocytes, endothelial cells, pancreatic beta cells, hepatocytes, skin cells, lung cells and skeletal muscle cells.

4. The scaffold of claim 2 , wherein a first of said at least two layers is seeded with cardiomyocytes and a second of said at least two layers is seeded with endothelial cells.

5. The scaffold of claim 1 , wherein a first of said at least two layers comprises a pattern of grooves and a second of said at least two layers comprises a pattern of grooves and cages.

6. The scaffold of claim 5 , wherein said cages are loaded with particles comprising an agent selected from the group consisting of a growth promoting agent, a differentiating agent, a neurotransmitter, a pro-angiogenic agent, an anti-inflammatory agent and a drug.

7. The scaffold of claim 1 , comprising at least three layers, wherein the third layer is patterned with cages loaded with particles comprising an agent selected from the group consisting of a growth promoting agent, a neurotransmitter, a differentiating agent, a pro-angiogenic agent, an anti-inflammatory agent and a drug.

8. The scaffold of claim 1 , wherein said at least two layers are adhered to one another using a biological glue.

Assignees

• Univ Ramot

Inventors

• Dvir Tal
• Fleischer Sharon

Classifications

• A61L27/54

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title

• A61L2300/41

  Anti-inflammatory agents, e.g. NSAIDs · CPC title

• A61L27/3808

  Endothelial cells · CPC title

• A61L27/56 Primary

  Porous materials, {e.g. foams or sponges} · CPC title

• A61L2430/20

  for reconstruction of the heart, e.g. heart valves · CPC title