Method of manufacturing nanostructure with improved cell adhesive ability containing fucoidan and nanostructure with improved cell adhesive ability containing fucoidan manufactured thereby

Patent metadata

Field	Value
Publication number	US-10968346-B2
Application number	US-201916299835-A
Country	US
Kind code	B2
Filing date	Mar 12, 2019
Priority date	Sep 13, 2016
Publication date	Apr 6, 2021
Grant date	Apr 6, 2021

Abstract

The present invention relates to a method of manufacturing a polycaprolactone nanostructure with improved cell adhesive ability containing fucoidan according to the present invention comprises dissolving fucoidan in glacial acetic acid as a solvent to obtain fucoidan-glacial acetic acid solution, mixing polycaprolactone with the fucoidan-glacial acetic acid solution to obtain a polycaprolactone-mixed solution, and manufacturing a nanostructure from the polycaprolactone-mixed solution by an electrospinning method. Therefore, a polycaprolactone nanostructure with improved cell adhesive ability containing fucoidan manufactured by the method according to the present invention exhibits characteristics of preventing fucoidan from being released from nanofibers by uniformly distributing fucoidan in the polycaprolactone nanostructure. Accordingly, the fucoidan-containing polycaprolactone nanostructure exhibits an effect capable of controlling cell activity while culturing adhered cells by facilitating adhesion of various types of cells.

First claim

What is claimed is:

1. A method of manufacturing a polycaprolactone nanostructure with improved cell adhesive ability containing fucoidan, the method comprising the steps of:
dissolving fucoidan in distilled water to obtain fucoidan-dissolved distilled water, and mixing the fucoidan-dissolved distilled water with a glacial acetic acid solution to obtain a fucoidan-glacial acetic acid solution;
dissolving polycaprolactone in the fucoidan-glacial acetic acid solution to obtain a solution;
stirring the solution at a temperature of 35 to 40° C. for 12 hours to obtain a resulting solution; and
manufacturing a nanostructure from the solution by an electrospinning method,
wherein, the fucoidan-glacial acetic acid solution obtained by the step of mixing the fucoidan-dissolved distilled water with a glacial acetic acid solution comprises 0.5 to 1 mg/ml of fucoidan.

2. The method of claim 1 , wherein the step of manufacturing a nanostructure from the solution by an electrospinning method comprises manufacturing a nanostructure from the solution by an electrospinning method at a voltage of 8 to 10 KV and a spinning speed of 8 to 10 μg/min.

3. A polycaprolactone nanostructure with improved cell adhesive ability containing fucoidan manufactured according to the method of claim 1 .

4. A polycaprolactone nanostructure with improved cell adhesive ability containing fucoidan manufactured according to the method of claim 2 .

Assignees

• Univ Ajou Ind Academic Coop Found

Inventors

• Kwak Jong Young
• Kim Jung Min
• Park Yeo Jin
• Park Dan Bi
• Jeong Young Hun
• Kim Jeong Hwa

Classifications

• D01D5/0061

  characterised by the electro-spinning apparatus · CPC title

• A61L2300/232

  Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides · CPC title

• C08J3/07

  from polymer solutions · CPC title

• A61L27/18 Primary

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds · CPC title

• A61L27/54

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title