High productivity algal mutants having reduced photosynthetic antenna

Patent metadata

Field	Value
Publication number	US-10968259-B2
Application number	US-201715859094-A
Country	US
Kind code	B2
Filing date	Dec 29, 2017
Priority date	Dec 30, 2016
Publication date	Apr 6, 2021
Grant date	Apr 6, 2021

Abstract

Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

First claim

What is claimed is:

1. A mutant Chlorophyte algal organism having an attenuated gene encoding an SGI1 polypeptide having at least 80% sequence identity to a polypeptide selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 14, SEQ ID NO: 15, and SEQ ID NO: 16; and
wherein the photosynthetic organism produces more biomass than a control organism grown or cultured under the substantially the same conditions, wherein the control photosynthetic organism does not have the attenuated SGI1 gene.

2. A mutant photosynthetic organism according to claim 1 , wherein the mutant photosynthetic organism produces at least 15% more lipid than a control photosynthetic organism grown or cultured under the substantially the same conditions.

3. A mutant photosynthetic organism according to claim 1 , wherein the mutant photosynthetic organism has reduced chlorophyll with respect to the control photosynthetic microorganism.

4. A mutant photosynthetic organism according to claim 3 , wherein the mutant photosynthetic organism has an increased chlorophyll a:b ratio with respect to the control photosynthetic organism.

5. A mutant photosynthetic organism according to claim 1 , wherein the mutant photosynthetic organism has a reduced photosystem II (PSII) antenna size with respect to the control photosynthetic organism.

6. A mutant photosynthetic organism according to claim 1 , wherein the mutant photosynthetic organism has a higher 14C Pmax with respect to the control photosynthetic organism.

7. A mutant photosynthetic organism according to claim 1 , wherein the mutant photosynthetic organism has a higher Fv/Fm than the control organism.

8. A mutant photosynthetic organism according to claim 1 , wherein the mutant photosynthetic organism has a higher protein content than the control organism.

9. A mutant photosynthetic organism according to claim 1 , wherein the mutant photosynthetic organism has a higher rubisco activase content than the control organism.

10. A mutant algal microorganism according to claim 1 , wherein the mutant algal microorganism is a Chlorophyte of the Class Chlorodendrophyceae, or Trebouxiophyceae.

11. A mutant algal microorganism according to claim 10 , wherein the mutant algal microorganism has a reduced photosystem II antenna size (PSII) antenna size with respect to the control microorganism.

12. A mutant algal microorganism according to claim 11 , wherein the cross-sectional unit size of the PSII antenna size is reduced by at least 25%, with respect to the cross-sectional unit size of the PSII antenna of the control microorganism.

13. A mutant algal microorganism according to claim 12 , wherein the mutant algal microorganism has increased Pmax (14C) with respect to the control algal microorganism.

14. A mutant algal microorganism according to claim 13 , wherein the Pmax (14C) is increased by at least 25% with respect to the Pmax (14C) of the control microorganism.

15. A mutant algal microorganism according to claim 1 , wherein the mutant algal microorganism has a reduced amount of chlorophyll b with respect to the control algal microorganism.

16. A mutant algal microorganism according to claim 1 , wherein the mutant algal microorganism has at least 20% less chlorophyll with respect to the control algal microorganism.

17. A mutant algal microorganism according to claim 1 , wherein the mutant algal microorganism has at least 20% less chlorophyll b with respect to the control algal microorganism.

18. A mutant algal microorganism according to claim 1 , wherein the mutant algal microorganism has a higher protein content than the control algal microorganism.

19. A mutant algal microorganism according to claim 18 , wherein the mutant algal microorganism has at least 10% more protein as a percent of total organic carbon than the control algal microorganism.

20. A mutant algal microorganism according to claim 19 , wherein the mutant algal microorganism has at least 20% more protein as a percent of total organic carbon than the control algal microorganism.

21. A mutant algal microorganism according to claim 1 , wherein the mutant algal microorganism has reduced expression of at least 10 LHC genes with respect to the control algal microorganism.

22. A mutant microorganism according to claim 1 , wherein the mutant algal microorganism has increased abundance of a ribulose bisphosphate carboxylase activator (RA) polypeptide with respect to the control algal microorganism.

23. A mutant microorganism according to claim 22 , wherein the ribulose bisphosphate carboxylase activator polypeptide is at least 1.5 times, at least 2 times, or at least 2.5 times as abundant in the mutant as in the control algal microorganism.

24. A mutant algal microorganism according to claim 1 , wherein the mutant algal microorganism is a Chlorophyte of the Class Chlorodendrophyceae, or Trebouxiophyceae.

25. A mutant algal microorganism according to claim 24 , wherein the mutant algal microorganism is a member of the Chlorophyceace, and optionally a species of a genus selected from the group consisting of Asteromonas, Ankistrodesmus, Carteria, Chlamydomonas, Chlorococcum, Chloro gonium, Chrysosphaera, Dunaliella, Haematococcus, Monoraphidium, Neochloris, Oedogonium, Pelagomonas, Pleurococcus, Pyrobotrys, Scenedesmus , and Volvox.

26. A mutant algal microorganism according to claim 24 , wherein the mutant algal microorganism is a member of the Class Chlorodendrophyceae, and optionally a species of a genus selected from the group consisting of Prasinocladus, Scherffelia , and Tetraselmis.

27. A mutant algal microorganism according to claim 24 , wherein the mutant algal microorganism is a member of the Prasinophyceae, optionally a species of Ostreococcus or Micromonas.

28. A mutant algal microorganism according to claim 24 , wherein the mutant algal microorganism is a member of the Class Trebouxiophyceae, and optionally a species of a genus selected from the group consisting of Botryococcus, Chlorella, Auxenochlorella, Heveochlorella, Marinichlorella, Parachlorella, Pseudochlorella, Tetrachlorella, Eremosphaera, Franceia, Micractinium, Nannochloris, Oocystis, Picochlorum, Prototheca, Stichococcus , and Viridiella.

29. A mutant algal microorganism according to claim 24 , wherein the mutant alga is of a genus selected from the group consisting of Chlorella , Auxenochlorella, Heveochlorella, Marinichlorella, Parachlorella, and Tetrachlorella.

30. A method of making an algal product comprising culturing the algal mutant of claim 1 to produce the product.

31. A method according to claim 30 , further comprising recovering the product from the culture.

32. A method according to claim 30 , wherein the product is biomass.

33. A method according to claim 30 wherein the algal product is one or more lipids, proteins, polyketides, terpenoids, pigments, antioxidants, vitamins, nucleotides, nucleic acids, amino acids, carbohydrates, alcohols, hormones, cytokines, peptides, or polymers.

34. A method according to claim 33 , wherein the product is one or more lipids.

35. A method according to claim 34 , wherein the product is triacylglyceride.

36. A method according to claim 33 , wherein the algal product is one or more proteins.

Assignees

• Synthetic Genomics Inc

Inventors

• Moellering Eric R
• Bauman Nicholas
• Radakovits Randor R
• Spreafico Roberto
• Kuzminov Fedor
• Ajjawi Imad
• Imam Saheed
• Schultz Andrew
• Kwok Kathleen
• Aqui Moena
• Nominati Jennifer
• Verruto John
• Bailey Shaun

Classifications

• C12R2001/89

  Algae {; Processes using algae} · CPC title

• C12N1/125

  Unicellular algae isolates · CPC title

• C12N1/36

  Adaptation or attenuation of cells · CPC title

• C07K14/405 Primary

  from algae · CPC title

• C12N1/12

  Unicellular algae; Culture media therefor (as new plants A01H13/00) · CPC title