Process for characterizing a blood sample

Patent metadata

Field	Value
Publication number	US-10962525-B2
Application number	US-201716347656-A
Country	US
Kind code	B2
Filing date	Nov 6, 2017
Priority date	Nov 7, 2016
Publication date	Mar 30, 2021
Grant date	Mar 30, 2021

Abstract

A process for characterizing a blood sample includes: insertion, into a channel with an inlet and outlet, of a solution including blood platelets, the solution being inserted via the inlet of the channel; creating a pressure difference between the inlet and the outlet to move the solution from the inlet towards the outlet of the channel; passage of the solution into a measurement zone of the channel, provided with electrodes; measurement, by the electrodes and measurement element, of an electric signal while the electrodes are covered by the solution in the channel. The progression of the solution from the inlet towards the outlet of the channel includes a progression of a front between, on the one hand, the solution extending from the front towards the inlet of the channel and, on the other hand, a gas extending from the front towards the outlet of the channel.

First claim

The invention claimed is:

1. Process for characterizing a blood sample, comprising:
introducing a solution comprising blood platelets into a channel ( 2 ), said channel comprising an inlet ( 3 ) and an outlet ( 4 ), the solution being introduced through the inlet of the channel,
creating a pressure difference between the inlet and the outlet of the channel, so as to move the solution forward from the inlet towards the outlet of the channel,
passing the solution into a measurement zone of the channel equipped with electrodes ( 8 ),
measuring an electrical signal using measurement means ( 10 ) and said electrodes, while said electrodes are covered with the solution in the channel,
wherein the forward movement of the solution from the inlet towards the outlet of the channel comprises a forward movement of a front between
the solution extending from the front towards the inlet of the channel, and
a gas extending from the front towards the outlet of the channel.

2. Process according to claim 1 , wherein, during the passage of the front through the inlet of the channel, the solution has a shear rate at the internal walls of the channel greater than 10,000 s −1 .

3. Process according to claim 1 , wherein, during the forward movement of the front from the inlet towards the outlet of the channel, the solution has a shear rate at the internal walls of the channel which decreases with time.

4. Process according to claim 1 , wherein during the passage of the front through the outlet of the channel, the solution has a shear rate at the internal walls of the channel less than 1000 s −1 .

5. Process according to claim 1 , wherein the pressure difference between the inlet and the outlet of the channel is constant during the forward movement of the solution in the channel.

6. Process according to claim 1 , wherein the channel has a cross-sectional area that is constant from inlet to outlet of the channel.

7. Process according to claim 1 , wherein the pressure difference between the inlet and the outlet of the channel is created by suction on the side with the outlet of the channel.

8. Process according to claim 1 , wherein the front has a forward movement velocity in the channel which decreases with time during the forward movement of the solution in the channel.

9. Process according to claim 1 , wherein the gas is air.

10. Process according to claim 1 , wherein the measurement of the electrical signal is carried out when the progression of the solution from the inlet towards the outlet of the channel is in a steady state, said channel being filled with the solution from the inlet to the outlet of the channel.

11. Process according to claim 1 , wherein the electrodes ( 8 ) are situated in a second half of the channel starting from the inlet of the channel.

12. Process according to claim 1 , further comprising, based on the measurement of the electrical signal, a calculation of an item of data relating to platelet discrimination.

13. Process according to claim 1 , wherein the measurement means impose a constant electrical current between the electrodes and measure a voltage between the electrodes while the electrical current is imposed.

14. Process according to claim 13 , further comprising an integration, over time, of the voltage measured.

15. Process according to claim 1 , further comprising:
detecting the passage of the front between the electrodes and the outlet of the channel, and/or
measuring a velocity of the solution in the channel, and calculating a viscosity of the solution as a function of the velocity measured, using technical processing means.

16. Process according to claim 1 , further comprising a temperature stabilization of the channel.

17. Process according to claim 1 , wherein the solution is a blood sample coming from a human or an animal, the process not comprising an addition of platelet activator to the blood sample as taken.

18. Process according to claim 1 , wherein, during the forward movement of the front from the inlet towards the outlet of the channel, the solution has a shear rate at the internal walls of the channel which decreases with time, until the front reaches the outlet of the channel.

19. Process according to claim 1 , wherein the pressure difference between the inlet and the outlet of the channel is constant during the forward movement of the solution in the channel, at least until the front reaches the outlet of the channel and/or up to the measurement.

20. Process according to claim 1 , wherein the front has a forward movement velocity in the channel which decreases with time during the forward movement of the solution in the channel, until the front reaches the outlet of the channel.

Assignees

• Univ Sorbonne
• Inst Nat Sante Rech Med
• Conception De Systemes Et Tech Mecanique Cstm

Inventors

• Rendu Francine
• Dufilho Monique
• Dugue François

Classifications

• G01N33/4905 Primary

  Determining clotting time of blood (by chemical methods G01N33/86, C12Q1/56) · CPC title

• G01N27/22

  by investigating capacitance · CPC title

• G01N33/50

  Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing (measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q) · CPC title

• G01N33/49

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title