Tat-induced crispr/endonuclease-based gene editing
US-2018073019-A1 · Mar 15, 2018 · US
Multiplexed genome editing
US10959413B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10959413-B2 |
| Application number | US-201615766551-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 7, 2016 |
| Priority date | Oct 8, 2015 |
| Publication date | Mar 30, 2021 |
| Grant date | Mar 30, 2021 |
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- Title
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Abstract
Official abstract text for this publication.
A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of inactivating expression of at least 20% of the copies of an endogenous retroviral target gene in a cell comprising multiple copies of the gene, comprising: (a) introducing into the cell (i) one or more guide ribonucleic acid (gRNA) sequences that comprise a portion that is complementary to a portion of the endogenous retroviral target gene, and (ii) a nucleic acid sequence that encodes a Cas protein; and (b) maintaining the cells under conditions in which (i) the Cas protein is expressed, (ii) the Cas protein and gRNAs form co-localization complexes with a plurality of the copies of the endogenous retroviral target gene, and (iii) the Cas protein cuts at least 20% of the copies of the endogenous retroviral target gene, thereby inactivating expression of said copies. 2. The method of claim 1 , wherein the cell is a stem cell, zygote, or a germ line cell. 3. The method of claim 1 , wherein the cell is a porcine cell. 4. The method of claim 1 , wherein the endogenous retroviral target gene is a porcine endogenous retrovirus (PERV) gene. 5. The method of claim 4 , wherein the PERV gene comprises a pol gene. 6. The method of claim 5 , wherein all copies of the pol gene in the cell are inactivated. 7. The method of claim 1 , wherein the Cas protein is a Cas9. 8. The method of claim 1 , wherein the one or more gRNA sequences are about 10 to about 1000 nucleotides.
Assignees
Inventors
Classifications
by genetic engineering · CPC title
- C12N15/8509Primary
for producing genetically modified animals, e.g. transgenic · CPC title
against retroviridae, e.g. HIV · CPC title
with deoxyribosyl as saccharide radical · CPC title
Animal producing cells or organs for transplantation · CPC title
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- What does patent US10959413B2 cover?
- A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas prote…
- Who is the assignee on this patent?
- Harvard College
- What technology area does this patent fall under?
- Primary CPC classification C12N15/8509. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 30 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).