Processes of producing fermentation products

Patent metadata

Field	Value
Publication number	US-10954533-B2
Application number	US-201916440623-A
Country	US
Kind code	B2
Filing date	Jun 13, 2019
Priority date	Mar 30, 2012
Publication date	Mar 23, 2021
Grant date	Mar 23, 2021

Abstract

The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and/or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.

First claim

The invention claimed is:

1. A process for producing a fermentation product from a starch-containing material comprising the steps of:
i) liquefying the starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase;
ii) saccharifying using a glucoamylase;
iii) fermenting using a yeast fermenting organism to produce the fermentation product; and
wherein a cellulolytic composition derived from a strain of Trichoderma , a strain of Humicola , or a strain of Chrysosporium , and comprising an endoglucanase, a beta-glucosidase, and a cellobiohydrolase is added during saccharification or fermentation.

2. The process of claim 1 , wherein the pH during liquefaction is from 4-7.

3. The process of claim 1 , wherein liquefying step i) is performed at a temperature between 70-95 degrees centigrade.

4. The process of claim 1 , wherein ii) saccharifying and iii) fermentation are done simultaneously.

5. The process of claim 1 , wherein the yeast is a Saccharomyces sp. yeast.

6. The process of claim 1 , wherein the yeast is a Saccharomyces cerevisiae yeast.

7. The process of claim 1 , wherein the cellobiohydrolase is cellobiohydrolase I (CBHI).

8. The process of claim 1 , wherein the cellobiohydrolase is a cellobiohydrolase II (CBHII).

9. The process of claim 1 , further wherein a trehalase is added during saccharification or fermentation.

10. The process of claim 1 , wherein the starch-containing material is corn.

11. The process of claim 1 , wherein the fermentation product is ethanol.

12. The process of claim 11 , wherein the ethanol yield is increased compared to the ethanol yield obtained without the cellulolytic composition.

Assignees

• Novozymes As

Inventors

• Rasmussen Thomas
• Saunders Jeremy
• Croonenberghs James
• Kang Zhengfang
• Craig Joyce
• Akerman Michael John

Classifications

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12P19/14 Primary

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12P7/06

  Ethanol, i.e. non-beverage · CPC title

• C12N9/2445

  Beta-glucosidase (3.2.1.21) · CPC title

• C12P7/14 Primary

  Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms · CPC title