Vessel for culturing human es cells
US-2015140652-A1 · May 21, 2015 · US
Cell culturing method
US10954487B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10954487-B2 |
| Application number | US-201716071969-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 20, 2017 |
| Priority date | Jan 21, 2016 |
| Publication date | Mar 23, 2021 |
| Grant date | Mar 23, 2021 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
Provided is a cell culturing method in which physical stress is reduced and oxygen can be supplied. The invention relates to a cell culturing method including; arranging cells in a dispersed state in a culture medium which is a plastic fluid; and introducing air bubbles into said culture medium.
First claim
Opening claim text (preview).
The invention claimed is: 1. A cell culturing method, comprising: arranging cells in a culture medium which is a plastic fluid so as to be suspended in the medium in a static state; culturing the cells in a suspended state; and introducing air bubbles into said culture medium and suppressing the rising speed of the air bubbles while maintaining the suspended state; wherein the cells are pluripotent stem cells or multipotent stem cells. 2. The cell culturing method according to claim 1 , wherein the culture medium which is a plastic fluid is a culture medium that can maintain the cells in the culture medium without allowing them to sediment in a static state. 3. The cell culturing method according to claim 1 , comprising culturing the cells using a cell culture vessel or a cell culture bag. 4. The cell culturing method according to claim 1 , wherein the air bubbles are introduced intermittently or continuously. 5. The cell culturing method according to claim 1 , wherein the air bubbles are microbubbles or submicrobubbles. 6. The cell culturing method according to claim 1 , wherein the culture medium has a yield value of at least 0.04 Pa.
Assignees
Inventors
Classifications
- C12M23/14Primary
Bags · CPC title
Culture process characterised by the use of mechanical forces, e.g. strain, vibration · CPC title
Substrates of biological origin, e.g. extracellular matrix, decellularised tissue · CPC title
Kinases (EC 2.7.) · CPC title
Collagen; Gelatin · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US10954487B2 cover?
- Provided is a cell culturing method in which physical stress is reduced and oxygen can be supplied. The invention relates to a cell culturing method including; arranging cells in a dispersed state in a culture medium which is a plastic fluid; and introducing air bubbles into said culture medium.
- Who is the assignee on this patent?
- Univ Osaka
- What technology area does this patent fall under?
- Primary CPC classification C12M23/14. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 23 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).