System, method, and device for high-throughput, automated culturing of genetically modified organisms

Patent metadata

Field	Value
Publication number	US-10954483-B2
Application number	US-201615557706-A
Country	US
Kind code	B2
Filing date	Mar 11, 2016
Priority date	Mar 12, 2015
Publication date	Mar 23, 2021
Grant date	Mar 23, 2021

Abstract

A fluid transfer system includes a transfer carousel capable of rotational and/or translational movement; at least one holding vessel (e.g. syringe) having a plunger, wherein the syringe is connected to the transfer carousel such that the movement of the transfer carousel results in movement of the syringe and wherein the syringe is capable of translational movement relative to the transfer carousel; a drive motor connected to the syringe that is capable of controlling the position of the plunger; and a peripheral module comprising at least one vessel that is capable of containing a fluid, wherein the vessel has an opening that can be mated with the syringe to allow fluid transfer between the vessel and the syringe. Methods for transferring a fluid are also disclosed.

First claim

We claim:

1. A closed, automated fluid transfer system comprising:
a transfer carousel capable of rotational and/or translational movement;
at least one syringe comprising a plunger, wherein the syringe is connected to the transfer carousel such that the movement of the transfer carousel results in movement of the syringe and wherein the syringe is capable of translational movement relative to the transfer carousel;
a drive motor connected to the syringe that is capable of controlling the position of the plunger, wherein the drive motor is partially or fully housed within the body of the syringe;
a peripheral module comprising an automated transfection station, wherein the automated transfection station comprises at least one vessel that is capable of containing a fluid, wherein the vessel has an opening that can be mated with the syringe to allow fluid transfer between the vessel and the syringe; and
an automated mobile platform, wherein the automated mobile platform is configured to bring together the syringe and the peripheral module to mate the opening of the transfection station vessel with the syringe.

2. The closed, automated fluid transfer system according to claim 1 , further comprising at least a second peripheral module.

3. The closed, automated fluid transfer system according to claim 2 , wherein the second peripheral module comprises a fluid loading station, wherein the fluid loading station comprises a loading vessel that is capable of containing a fluid, is capable of rotational and/or translational movement, and can be mated with the syringe to allow fluid transfer between the fluid loading station and the syringe.

4. The closed, automated fluid transfer system according to claim 3 , further comprising a fluid supply rig, wherein the fluid supply rig comprises one or more supply vessels capable of containing a fluid, which can be mated with the fluid loading station to allow fluid transfer between the supply vessel and the fluid loading station.

5. The closed, automated fluid transfer system according to claim 4 , wherein the fluid supply rig is capable of rotational or translational movement.

6. The closed, automated fluid transfer system according to claim 2 , wherein the second peripheral module further comprises an analysis station.

7. The closed, automated fluid transfer system according to claim 3 , wherein the second peripheral module further comprises a centrifuge comprising a centrifuge vessel capable of containing a fluid, wherein the fluid loading station is configured to be rotated or translated and/or the centrifuge is configured to be translated or rotated to mate the loading vessel with the centrifuge vessel to allow fluid transfer between the loading vessel and the centrifuge vessel.

8. The closed, automated fluid transfer system according to claim 3 , further comprising a waste station, wherein the fluid loading station can be rotated or translated to mate the fluid loading station with the waste station to allow fluid transfer between the loading station and the waste station.

9. The closed, automated fluid transfer system according to claim 3 , further comprising a chromatography rig, wherein the fluid loading station can be rotated or translated to mate the fluid loading station with the chromatography rig to allow fluid transfer between the loading station and the chromatography rig.

10. The closed, automated fluid transfer system according to claim 1 , further comprising at least one motor wherein the motor controls the movement of the transfer carousel.

11. The closed, automated fluid transfer system according to claim 1 , wherein the automated transfection station comprises an automated electroporation system.

12. The closed, automated fluid transfer system according to claim 6 , wherein the analysis station comprises an imaging device from which data may be obtained and used to perform one or more analytical techniques selected from Partial Least Squares Regression, Gaussian Process Regression, and Support Vector Machines to estimate one or more process parameters selected from cell viability, cell density, and titer of target compounds.

13. The closed, automated fluid transfer system according to claim 6 , wherein the analysis station comprises a fluorescence detector, which is capable of collecting data that can be used to estimate process parameters selected from cell viability, cell density, and titer of target compounds for organisms that express fluorescent markers.

14. The closed, automated fluid transfer system according to claim 1 , wherein the peripheral module is further configured to assemble a DNA sequence from a plurality of modular parts according to an encoded plan.

15. The closed, automated fluid transfer system according to claim 2 , wherein the mobile platform can bring together the syringe and the second peripheral module.

16. The closed, automated fluid transfer system according to claim 1 , wherein the syringe further comprises miniaturized analytical instrumentation embedded in the plunger that is configured to monitor contents of the syringe.

17. The closed, automated fluid transfer system according to claim 1 , wherein the syringe further comprises one or more ports that pass through the plunger and permit monitoring of gas tension in contents of the syringe and/or control of gas tension in the contents of the syringe.

Assignees

• Univ Pennsylvania

Inventors

• Hogan Michael
• Telhan Orkan M
• Hogan Karen

Classifications

• C12M23/44 Primary

  Multiple separable units; Modules · CPC title

• B01J2219/0029

  with pistons or plungers · CPC title

• B01J2219/00376

  in multiple or parallel arrangements · CPC title

• B01J2219/00326

  Movement by rotation · CPC title

• B01J2219/00353

  Pumps · CPC title