Imaging mass spectrometry data processing device and imaging mass spectrometry data processing method

Patent metadata

Field	Value
Publication number	US-10950423-B2
Application number	US-201616328122-A
Country	US
Kind code	B2
Filing date	Aug 26, 2016
Priority date	Aug 26, 2016
Publication date	Mar 16, 2021
Grant date	Mar 16, 2021

Abstract

The user specifies regions of interest (ROIs) such as a region where a large amount of compound to be identified is estimated to be included and a region where the compound is overlapped with another compound on one or more specific MS images, and specifies addition or subtraction of the ROIs. For each of the specified ROIs, an average MS/MS spectrum is calculated from MS/MS spectrum data at measurement points in the regions, and the average MS/MS spectra at the ROIs are subjected to addition or subtraction, to obtain an MS/MS spectrum. By addition between the ROIs, the intensity of peak derived from the target compound can be increased. By subtraction between the ROIs, a peak derived from the other compound overlapped with the target compound can be removed. When the MS/MS spectrum after addition or subtraction is subjected to library search for identification, a score indicating the similarity of the spectrum is higher than the conventional score, and the identification accuracy can be improved.

First claim

The invention claimed is:

1. An imaging mass spectrometry data processing device for processing MS n spectrum data obtained by MS n analysis (n is an integer of two or more) at each of a plurality of measurement points within a predetermined region to be measured on a sample, comprising:
a) an image creation unit for creating a mass spectrometry image illustrating signal intensity distribution at a specific mass-to-charge ratio of the region to be measured or a part of the region to be measured based on the MS n spectrum data;
b) a region-of-interest setting unit for setting a plurality of small regions as regions of interest on the mass spectrometry image or on an optical image corresponding to the region to be measured, wherein the regions of interest include a plurality of measurement points;
c) an MS n spectrum acquisition unit for
acquiring an average MS n spectrum of each of the plurality of regions of interest by averaging MS n spectra of the plurality of measurement points in the each of the plurality of region of interest, or determining a typical MS n spectrum of each of the plurality of regions of interest among MS n spectra of the plurality of measurement points in the each of the plurality of region of interest, and
acquiring a calculated MS n spectrum by addition or subtraction of the average MS n spectra or the typical MS n spectra at the plurality of regions of interest; and

d) a compound identification unit for identifying a compound existing in the plurality of regions of interest based on similarity between the calculated MS n spectrum and MS n spectra stored in a spectrum library.

2. The imaging mass spectrometry data processing device according to claim 1 , further comprising:
an image display processing unit for displaying the mass spectrometry image or the optical image on a screen of a display unit; and
a region-of-interest specifying unit for specifying an optional small region as a region of interest on the displayed mass spectrometry image or optical image by a user, wherein
the region-of-interest setting unit sets the small region specified by the region-of-interest specifying unit for the region of interest.

3. The imaging mass spectrometry data processing device according to claim 1 , further comprising:
a reference image creation unit for creating a plurality of reference mass spectrometry images illustrating signal intensity distribution at a plurality of main mass-to-charge ratios based on the MS n spectrum data;
an image classification unit for classifying the plurality of reference mass spectrometry images into one or more groups based on similarity of signal intensity distribution; and
a reference image display processing unit for displaying the classified reference mass spectrometry images on a screen of a display unit.

4. The imaging mass spectrometry data processing device according to claim 3 , wherein
the image classification unit classifies the reference mass spectrometry images into one or more groups using principal component analysis.

5. The imaging mass spectrometry data processing device according to claim 3 , wherein
the reference image display processing unit displays on the screen of the display unit an image obtained by coloring typical reference mass spectrometry images in a plurality of classified groups with different colors and overlapping the images, and causes the region-of-interest setting unit to set a region of interest based on the image.

6. The imaging mass spectrometry data processing device according to claim 1 , wherein
the compound identification unit refers to a library including the MS n spectra of known compounds, and performs compound identification,
the compound identification unit stores an MS n spectrum of a mixture containing one or more compounds to be mixed with the known compounds in the library with a mixing condition for the mixture, and
when a part of analysis conditions during acquiring MS n spectrum data to be processed is consistent with the mixing condition, the MS n spectrum in the library corresponding to the mixing condition is subtracted from an actually measured MS n spectrum and library search is performed.

7. The imaging mass spectrometry data processing device according to claim 1 , wherein
the compound identification unit refers to the library including the MS n spectra of known compounds, and performs compound identification, and
the compound identification unit performs compound identification based on similarity between an MS n spectrum obtained by combining the plurality of MS n spectra stored in the library and the actually measured MS n spectrum.

8. The imaging mass spectrometry data processing device according to claim 1 , wherein
the compound identification unit refers to the library including the MS n spectra of known compounds, and performs compound identification, and
the compound identification unit performs compound identification based on similarity between an MS n spectrum obtained by shifting each peak on the MS n spectra stored in the library upwardly or downwardly by a predetermined mass-to-charge ratio and the actually measured MS n spectrum.

9. An imaging mass spectrometry data processing method for processing MS n spectrum data obtained by MS n analysis (n is an integer of two or more) at each of a plurality of measurement points within a predetermined region to be measured on a sample, comprising:
an image creation step of creating a mass spectrometry image illustrating signal intensity distribution at a specific mass-to-charge ratio of the region to be measured or a part of the region to be measured based on the MS n spectrum data;
a region-of-interest setting step of setting a plurality of small regions as a plurality of regions of interest on the mass spectrometry image or an optical image corresponding to the region to be measured, wherein the regions of interest include a plurality of measurement points;
an MS n spectrum acquisition step of
acquiring an average MS n spectrum of each of the plurality of regions of interest by averaging MS n spectra of the plurality of measurement points in the each of the plurality of region of interest, or determining a typical MS n spectrum of each of the plurality of regions of interest among MS n spectra of the plurality of measurement points in the each of the plurality of region of interest, and
acquiring a calculated MS n spectrum obtained by addition or subtraction of the average MS n spectra or the typical MS n spectra at the plurality of regions of interest; and

a compound identification step of identifying a compound existing in the plurality of regions of interest using the calculated MS n spectrum.

10. The imaging mass spectrometry data processing device according to claim 4 , wherein
the reference image display processing unit displays on the screen of the display unit an image obtained by coloring typical reference mass spectrometry images in a plurality of classified groups with different colors and overlapping the images, and causes the region-of-interest setting unit to set a region of interest based on the image.

Assignees

• Shimadzu Corp

Inventors

• Ikegami Masahiro
• Ogata Koretsugu

Classifications

• H01J49/0004 Primary

  Imaging particle spectrometry · CPC title

• H01J49/0036

  Step by step routines describing the handling of the data generated during a measurement · CPC title

• H01J49/027 Primary

  detecting image current induced by the movement of charged particles (H01J49/38 takes precedence) · CPC title

• H01J49/004

  Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn · CPC title

• G01N27/62

  by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode · CPC title