Device and method for capturing images

Patent metadata

Field	Value
Publication number	US-10948705-B2
Application number	US-201616065263-A
Country	US
Kind code	B2
Filing date	Dec 22, 2016
Priority date	Dec 23, 2015
Publication date	Mar 16, 2021
Grant date	Mar 16, 2021

Abstract

A device for recording images is provided, an image-recording device and an illumination device being arranged on the same side of a specimen plane in said device. The image-recording device has illumination portions, for example individual light sources, which are actuatable independently of one another in order to be able to illuminate a specimen in the specimen plane at different angles and/or from different directions. In this way, it is possible to record a plurality of images with different illuminations, which can be combined to form a results image with improved properties.

First claim

The invention claimed is:

1. A device for recording images, comprising:
an image-recording device;
an illumination device arranged around an optical axis of the image-recording device;
a further image-recording device; and
at least one of a further illumination device that couples light into a beam path of the image-recording device, or an additional illumination device that couples the light into a beam path of the further image-recording device,
wherein the illumination device has a cut-out to facilitate a passage of light to the image-recording device,
wherein the illumination device has a plurality of independently actuatable illumination portions,
wherein the image-recording device and the illumination device are arranged on a same side of a specimen plane of the image-recording device,
wherein, with respect to the image-recording device, the further image-recording device is on an opposite side of the specimen plane,
wherein an angle between adjacent illumination portions of the illumination device as seen from the specimen plane is less than or equal to an angle defined by a numerical aperture of the image-recording device, and
wherein the further image-recording device differs from the image-recording device with respect to at least one of numerical aperture, working distance, magnification or resolution.

2. The device of claim 1 , wherein the angle between adjacent illumination portions of the illumination device is at least 10% smaller than the angle defined by the numerical aperture of the image-recording device.

3. The device of claim 1 ,
wherein the plurality of illumination portions comprise a plurality of light source elements, and
wherein the light source elements are actuatable individually and/or segment-by-segment.

4. The device of claim 3 , wherein different light source elements have different properties in respect of a spectral range and/or polarization.

5. The device of claim 1 , wherein the illumination device comprises an areal light source and selectively actuatable shadowing elements for dimming respective parts of the areal light source.

6. The device of claim 1 , wherein the illumination device is ring-shaped with an internal diameter (Rin) and an external diameter (Rout), wherein the internal diameter (Rin) defines the cut-out.

7. The device of claim 1 , wherein the further illumination device comprises at least one of a movable light source element or a plurality of independently actuatable further illumination portions.

8. The device of claim 1 , further comprising:
a control device,
wherein the control device is configured to actuate the illumination device sequentially for activating various illumination portions in order to illuminate a specimen in the specimen plane at different angles and/or from different directions and to actuate the image-recording device in order to obtain a corresponding plurality of images for various actuations of the illumination device.

9. The device of claim 8 , wherein the control device is further configured to combine the plurality of images to form a result image.

10. The device of claim 9 , wherein the control device is configured to carry out one or more of the following when combining the plurality of images to form a result images:
a reflection correction,
an improvement in the resolution,
an improvement in contrast,
a determination of a z-map of the specimen,
a determination of a phase contrast image, or
an improvement in a depth-of-field range.

11. The device of claim 9 , wherein the control device is further configured to evaluate a quality of the result image and, depending on the evaluation, to modify the sequential actuation of the illumination device and/or a selection of images to be combined.

12. A method for recording a plurality of images using a device comprising:
an image-recording device;
an illumination device arranged around an optical axis of the image-recording device;
a further image-recording device; and
at least one of a further illumination device that couples light into a beam path of the image-recording device, or an additional illumination device that couples the light into a beam path of the further image-recording device,
wherein the illumination device has a cut-out to facilitate a passage of light to the image-recording device,
wherein the illumination device has a plurality of independently actuatable illumination portions,
wherein the image-recording device and the illumination are arranged on a same side of a specimen plane of the image-recording device;
wherein, with respect to the image-recording device, the further image-recording device is on an opposite side of the specimen plane,
wherein an angle between adjacent illumination portions of the illumination device as seen from the specimen plane is less than or equal to an angle defined by a numerical aperture of the image-recording device, and
wherein different illumination portions of the illumination device of the device are activated for various images of the plurality of images,
the method comprising:
combining the plurality of images to form a result image with improved image properties.

Assignees

• Zeiss Carl Microscopy Gmbh

Inventors

• Gaiduk Alexander
• Sticker Markus
• Stoppe Lars
• Wolleschensky Ralf

Classifications

• G02B21/10

  affording dark-field illumination (G02B21/14 {and G02B21/125} take precedence) · CPC title

• G02B21/084

  having annular illumination around the objective · CPC title

• G02B21/125 Primary

  affording both dark- and bright-field illumination · CPC title

• G02B21/0064

  multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling (G02B21/0076 takes precedence) · CPC title

• G02B27/1066

  for enhancing image performance, like resolution, pixel numbers, dual magnifications or dynamic range, by tiling, slicing or overlapping fields of view · CPC title