Mass spectrometry assay for congenital adrenal hyperplasia

Patent metadata

Field	Value
Publication number	US-10948501-B2
Application number	US-202016915729-A
Country	US
Kind code	B2
Filing date	Jun 29, 2020
Priority date	Dec 24, 2008
Publication date	Mar 16, 2021
Grant date	Mar 16, 2021

Abstract

Methods are provided for detecting the amount of one or more CAH panel analytes (i.e., pregnenolone, 17-OH pregnenolone, progesterone, 17-OH progesterone, dehydroepiandrosterone (DHEA), androstenedione, testosterone, deoxycorticosterone, 11-deoxycortisol, and cortisol) in a sample by mass spectrometry. The methods generally involve ionizing one or more CAH panel analytes in a sample and quantifying the generated ions to determine the amount of one or more CAH panel analytes in the sample. In methods where amounts of multiple CAH panel analytes are detected, the amounts of multiple analytes are detected in the same sample injection.

First claim

That which is claimed is:

1. A method for determining the amount of 17-OH pregnenolone in a sample by mass spectrometry, the method comprising:
(i) purifying the sample by liquid chromatography;
(ii) ionizing the sample under conditions suitable to produce one or more 17-OH pregnenolone ions detectable by mass spectrometry, wherein the one or more 17-OH pregnenolone ions comprise one or more ions having a m/z of about 297.2±0.5, 105.6±0.5, and/or 91.1±0.5;
(iii) determining the amount of the one or more 17-OH pregnenolone ions by mass spectrometry, wherein the method has a limit of quantitation of 6 ng/dL; and
(iv) using the determined amount of the one or more ions to determine the amount of 17-OH pregnenolone in the sample.

2. The method of claim 1 , wherein said liquid chromatography is high performance liquid chromatography, ultra high performance liquid chromatography, or turbulent flow liquid chromatography.

3. The method of claim 1 , wherein the sample has been purified by solid phase extraction prior to ionization.

4. The method of claim 1 , further comprising adding internal standard to the sample, wherein an internal standard is used for determination of the amount of 17-OH pregnenolone in the sample.

5. The method of claim 4 , wherein the internal standard comprises an isotopically labeled analog of 17-OH pregnenolone.

6. The method of claim 1 , wherein the sample comprises plasma or serum.

7. The method of claim 1 , wherein the ionizing comprises ionization by electrospray ionization (ESI).

Assignees

• Quest Diagnostics Invest Inc

Inventors

• Ghoshal Amit
• Clarke Nigel J
• Goldman Mildred M

Classifications

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title

• H01J49/165

  Electrospray ionisation · CPC title

• G01N2800/04

  Endocrine or metabolic disorders · CPC title

• C12Q1/6872

  involving mass spectrometry · CPC title

• G01N33/689

  related to pregnancy or the gonads · CPC title