Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
US-10494656-B2 · Dec 3, 2019 · US
Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
US10947572B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10947572-B2 |
| Application number | US-202017002671-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 25, 2020 |
| Priority date | May 11, 2012 |
| Publication date | Mar 16, 2021 |
| Grant date | Mar 16, 2021 |
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- Title
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- Abstract
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Abstract
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The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
First claim
Opening claim text (preview).
What is claimed is: 1. An engineered polypeptide having imine reductase activity, wherein said polypeptide has at least 90% sequence identity to SEQ ID NO:2, and comprises a substitution at a position corresponding to position 259 of SEQ ID NO:2, wherein the amino acid at position 259 has been replaced with a non-polar, aliphatic, polar, or aromatic residue. 2. The engineered polypeptide having imine reductase activity of claim 1 , wherein said polypeptide has at least 90% sequence identity to SEQ ID NO:2, wherein the amino acid at the position corresponding to position 259 of SEQ ID NO:2 has been replaced with histidine. 3. The engineered polypeptide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1a) pyruvate, and substrate compound (2b) butylamine to product compound (3b), N-2-(butylamino)propanoic acid, under suitable reaction conditions. 4. The engineered polypeptide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1b) cyclohexanone, and substrate compound (2a) L-norvaline to product compound (3c), (S)-2-(cyclohexylamino)pentanoic acid, under suitable reaction conditions. 5. The engineered polypeptide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1b) cyclohexanone, and substrate compound (2b) butylamine to product compound (3d), N-butylcyclohexanamine, under suitable reaction conditions. 6. The engineered polypeptide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1i), and substrate compound (2b) to product compound (3n), under suitable reaction conditions. 7. The engineered polypeptide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1j), and substrate compound (2b) to product compound (3o), under suitable reaction conditions.
Assignees
Inventors
Classifications
N5-(Carboxyethyl)ornithine synthase (1.5.1.24) · CPC title
Opine dehydrogenase (1.5.1.28) · CPC title
Heterocyclic compounds containing sulfur atoms as ring hetero atoms in the condensed system (cepam nucleus C12P35/00; penam nucleus C12P37/00) · CPC title
Alanine; Leucine; Isoleucine; Serine; Homoserine · CPC title
Tauropine dehydrogenase (1.5.1.23) · CPC title
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Frequently asked questions
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- What does patent US10947572B2 cover?
- The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0028. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 16 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 11 related publications on this page (citations in our corpus or others sharing the same primary CPC).