Hexuronate C4-epimerase mutant with improved conversion activity, and method for producing D-tagatose by using same
US-10544409-B2 · Jan 28, 2020 · US
Hexuronate C4-epimerase variant having improved D-tagatose conversion activity, and D-tagatose production method using same
US10947524B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10947524-B2 |
| Application number | US-201716060018-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 31, 2017 |
| Priority date | Jul 29, 2016 |
| Publication date | Mar 16, 2021 |
| Grant date | Mar 16, 2021 |
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Abstract
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Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.
First claim
Opening claim text (preview).
The invention claimed is: 1. A hexuronate C4-epimerase variant in which serine (S) as an amino acid residue at position 185 from the N-terminus of a hexuronate C4-epimerase consisting of the amino acid sequence set forth in SEQ ID NO: 1 is replaced by alanine (A), glycine (G), histidine (H), lysine (K), glutamine (Q) or arginine (R). 2. The hexuronate C4-epimerase variant according to claim 1 , wherein the serine (S) residue at position 125 from the N-terminus of the hexuronate C4-epimerase is further replaced by cysteine (C), tyrosine (Y), glutamine (Q), glutamic acid (E), threonine (T), asparagine (N) or aspartic acid (D). 3. The hexuronate C4-epimerase variant according to claim 2 , wherein the serine (S) residue at position 268 from the N-terminus of the hexuronate C4-epimerase is further replaced by cysteine (C) or threonine (T). 4. A composition for D-tagatose production comprising the hexuronate C4-epimerase variant according to claim 1 .
Assignees
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Classifications
produced by the action of an isomerase, e.g. fructose · CPC title
Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title
- C12N9/90Primary
Isomerases (5.) · CPC title
acting on hydroxy acids and derivatives (5.1.2) · CPC title
acting on carbohydrates and derivatives (5.1.3) · CPC title
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- What does patent US10947524B2 cover?
- Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.
- Who is the assignee on this patent?
- Cj Cheiljedang Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N9/90. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 16 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).