Method of preparing ultra-low molecular weight keratin peptide

Patent metadata

Field	Value
Publication number	US-10945940-B2
Application number	US-201615771747-A
Country	US
Kind code	B2
Filing date	Oct 31, 2016
Priority date	Oct 29, 2015
Publication date	Mar 16, 2021
Grant date	Mar 16, 2021

Abstract

The present disclosure relates to methods of preparing an ultra-low molecular weight keratin peptide and use thereof. In particular, the present disclosure relates to a method of preparing an ultra-low molecular weight keratin peptide using culturing a microorganism having keratinolytic activity in a medium including keratin, ultrafiltration, ion exchange chromatography and gel filtration chromatography, a peptide prepared by the method, and a cosmetic and food composition for preventing or improving skin aging or skin wrinkles including the same. According to the method of preparing a keratin peptide of the present invention, it is possible to eco-friendly biologically treat waste resources and efficiently purify and recover anti-aging functional ultra-low molecular weight keratin peptides. In addition, the keratin peptide of the present disclosure breaks down collagen to have abilities to inhibit MMP-1 expression and activity, which is an enzyme that causes skin aging, which has an excellent effect on anti-skin aging and skin wrinkle improvement and has no toxicity to skin cells. It is suitable for use as a cosmetic, pharmaceutical or food composition for preventing, improving or treating skin aging or skin wrinkles, thereby being effectively used for the efficient and rapid production and development of high value-added functional cosmetic substances.

First claim

What is claimed is:

1. A method for preventing or improving skin aging or skin wrinkles, the method comprising the step of treating a skin of subject with a cosmetic composition comprising a peptide comprising at least one sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 9.

2. The method of claim 1 , wherein the peptide is prepared by a method comprising the steps of:
(1) obtaining keratin hydrolysates by culturing a microorganism having keratinolytic activity in a culture medium including keratin;
(2) fractionating a protein having a molecular weight of 100 Da to 1000 Da in the keratin hydrolysates obtained from step (1);
(3) purifying the protein by performing ion exchange chromatography, gel filtration chromatography or a combination thereof on the fractionated product obtained from step (2); and
(4) purifying and desalting the protein by performing gel filtration chromatography on the purified protein obtained from step (3).

3. The method of claim 2 , wherein the microorganism in step (1) is Fervidobacterium islandicum AW-1 (KCTC4680).

4. The method of claim 2 , wherein the keratin in step (1) is derived from a feather, hair, leather, a nail, a claw, a horn or a hoof of a bird or a mammal.

5. The method of claim 2 , wherein the keratin in step (1) is derived from a feather of a bird.

6. The method of claim 5 , wherein the feather is present in the culture medium in an amount of 5 g/L to 15 g/L.

7. The method of claim 2 , wherein the culture in step (1) is an anaerobic culture.

8. The method of claim 2 , wherein the culture in step (1) is a static anaerobic culture.

9. The method of claim 2 , wherein the keratin peptide has an ability to inhibit matrix metalloproteinase-1 (MMP-1) expression or ability to inhibit MMP-1 activity.

10. The method of claim 2 , wherein the culture in step (1) is an anaerobic culture performed under a temperature condition of 60° C. to 90° C.

11. The method of claim 2 , wherein the fraction in step (2) is performed by ultrafiltration.

12. The method of claim 1 , wherein the skin aging is caused by ultraviolet rays.

13. The method of claim 1 , wherein the skin wrinkles is caused by ultraviolet rays.

14. The method of claim 1 , wherein the cosmetic composition comprising a peptide comprises SEQ ID NO: 9.

15. The method of claim 1 , wherein the cosmetic composition comprises a first peptide comprising SEQ ID NO: 9 and a second peptide selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 8.

Assignees

• Kyungpook Nat Univ Ind Academic Coop Found

Inventors

• Lee Dong Woo
• Kang Nam Joo
• Lee Yong Jik
• Jin Hyeon Su
• Yeo In Hyuk
• Song Kyeong Seop
• Lee Jae-Eun

Classifications

• C12P21/06

  produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00) · CPC title

• B01D15/34

  Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title

• A23L33/18

  Peptides; Protein hydrolysates · CPC title

• C07K1/18

  Ion-exchange chromatography · CPC title