Parallel assembly of chromatography column modules

Patent metadata

Field	Value
Publication number	US-10940403-B2
Application number	US-201815954545-A
Country	US
Kind code	B2
Filing date	Apr 16, 2018
Priority date	Jun 3, 2010
Publication date	Mar 9, 2021
Grant date	Mar 9, 2021

Abstract

A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b) connected in a rigid housing (21; 61), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules in the rigid housing are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

First claim

The invention claimed is:

1. A chromatography assembly, comprising:
one or more chromatography column modules, each chromatography column module comprising; (i) a tube defined by a plurality of columns; (ii) an upper piece and a lower piece, together with the tube, defining a bed space configured to hold a chromatography medium; and (iii) a plurality of fluid conduits configured to fluidly connect to another chromatography module at a plurality of connections points arranged on a top side and a bottom side of the bed space;
a housing arranged to house the one or more chromatography column modules and comprising a first end plate and a second end plate mounted on opposite ends of the housing, such that the one or more chromatography column modules is positioned between the first and second end plates;
an inlet provided in the first end plate; and an outlet provided in the second end plate;
wherein, when a first chromatography column module is connected to a second chromatography column module within the housing, the plurality of fluid conduits is adapted to connect (i) the bed space of the first chromatography module to the bed space of the second chromatography column module and (ii) the bed space of the first and second chromatography column modules to the inlet and the outlet; and
wherein, the total length and/or volume of the fluid conduit from the inlet to the bed space together with the length and/or volume of the fluid conduit from the bed space to the outlet of the first chromatography column module is substantially the same for all connected chromatography column modules;
wherein the chromatography assembly further comprises two fluid distribution modules, one arranged at each end of a plurality of column modules between the end most module and a respective end piece, each distribution module having a fluid connection providing an alternative assembly inlet or assembly outlet of the assembly and a connection face oriented towards the side by side column modules, the connection face having at least two connection face fluid connections for connecting to the common inlet or common outlet of chromatography column modules.

2. The assembly according to claim 1 , wherein the first and second end plates form part of the housing containing the chromatography column modules.

3. The assembly according to claim 2 , wherein the housing further includes tie bars which together with the first and second end plates forms a compression frame.

4. The assembly according to claim 3 , wherein a compression three is applied to the one or more chromatography column modules to form fluid tight connections therebetween.

5. The assembly according to claim 1 , wherein the total length and/or volume of the fluid conduits from the inlet to a respective module volume together with the length and/or volume of the fluid conduits from the same module volume to the outlet is substantially the same for all module volumes of the assembly.

6. The chromatography column module adapted to be used side by side in an assembly according to claim 1 , wherein parallel separation is provided between the chromatography column module along with another chromatography column module.

7. The chromatography column module according to claim 6 , wherein seals are provided to each adjoining face the of the chromatography column modules.

8. The chromatography column module according to claim 7 , characterized in that aseptic films are provided over said seals.

9. The chromatography column module according to claim 6 , wherein the volume is partially prefilled with dry chromatography gel in an amount such that when liquid is added to the volume the dry gel will swell to be compressed in the volume.

10. The chromatography column module according to claim 6 , including a sensor.

11. The chromatography column module according to claim 6 , including an adjustable flow restrictor.

Assignees

• Cytiva BioPro0cess R&D AB
• Cytiva Bioprocess R & D Ab

Inventors

• Gebauer Klaus
• Ramakrishna Manoj Kumar
• Salomonsson Daniel
• Lofving Per

Classifications

• B01D15/22 Primary

  relating to the construction of the column · CPC title

• G01N30/6004

  end pieces · CPC title

• G01N30/466

  with separation columns in parallel · CPC title

• F16L55/00

  Devices or appurtenances for use in, or in connection with, pipes or pipe systems (F16L1/00 - F16L53/00, F16L57/00, F16L59/00 take precedence; repairing or joining pipes on or under water F16L1/26; cleaning of pipes B08B9/02, e.g. removal of blockages B08B9/027; devices for preventing bursting of water pipes by freezing E03B7/10) · CPC title

• Y10T29/49826

  Assembling or joining · CPC title