Recombinant expression vector applicable to rapid screening for recombinant strain and application
US-12037632-B2 · Jul 16, 2024 · US
Thermostable cellulases
US10934537B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10934537-B2 |
| Application number | US-201615577641-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 27, 2016 |
| Priority date | May 28, 2015 |
| Publication date | Mar 2, 2021 |
| Grant date | Mar 2, 2021 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The invention further relates to polypeptides encoded by such a nucleic acid molecule, to constructs, vectors and cells comprising the nucleic acid molecule and to a use and method of using a polypeptide of the invention for degrading cellulose.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of degrading any one or more of cellulose, hemicellulose, lichenin and cereal beta-D-glucan or a material comprising cellulose, hemicellulose, lichenin and/or cereal beta-D-glucan, comprising incubating a polypeptide with said cellulose, hemicellulose, lichenin and/or cereal beta-D-glucan or a material comprising cellulose, hemicellulose, lichenin and/or cereal beta-D-glucan, wherein said polypeptide has a cellulase activity, is capable of degrading cellulose to glucose and is thermostable, and said polypeptide comprises: the amino acid sequence set forth in SEQ ID NO. 2, or an amino acid sequence having at least 80% sequence identity to the entirety of SEQ ID NO. 2. 2. The method of claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 95% sequence identity to the entirety of SEQ ID NO. 2. 3. The method of claim 2 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO. 2. 4. A method of degrading any one or more of cellulose, hemicellulose, lichenin and cereal beta-D-glucan or a material comprising cellulose, hemicellulose, lichenin and/or cereal beta-D-glucan, comprising incubating a polypeptide with said cellulose, hemicellulose, lichenin and/or cereal beta-D-glucan or a material comprising cellulose, hem icellulose, lichenin and/or cereal beta-D-glucan, wherein said polypeptide has a cellulase activity and is thermostable, and said polypeptide comprises the amino acid sequence set forth in SEQ ID NO. 8, or an amino acid sequence having at least 80% sequence identity to the entirety of SEQ ID NO. 8. 5. The method of claim 4 , wherein the polypeptide comprises an amino acid sequence having at least 95% sequence identity to the entirety of SEQ ID NO. 8. 6. The method of claim 5 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO. 8. 7. The method of claim 5 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO. 10. 8. The method of claim 5 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO. 12. 9. The method of claim 1 , wherein the material comprising cellulose, hem icellulose, lichenin and/or cereal beta-D-glucan is lignocellulose. 10. The method of claim 1 , further comprising production of a biofuel. 11. The method of claim 1 , wherein a part of said amino acid sequence having at least 80% sequence identity to the entirety of SEQ ID NO. 2 has at least 90% sequence identity to the entirety of SEQ ID NO. 20. 12. The method of claim 11 , wherein a first part of said amino acid sequence having at least 80% sequence identity to the entirety of SEQ ID NO. 2 has at least 90% sequence identity to the entirety of SEQ ID NO. 18, and a second part of said amino acid sequence having at least 80% sequence identity to the entirety of SEQ ID NO. 2 has at least 90% sequence identity to the entirety of SEQ ID NO. 20. 13. The method of claim 4 , wherein the material comprising cellulose, hemicellulose, lichenin and/or cereal beta-D-glucan is lignocellulose. 14. The method of claim 4 , further comprising production of a biofuel.
Assignees
Inventors
Classifications
Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title
produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title
Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title
- C12N9/2437Primary
Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150) · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US10934537B2 cover?
- The invention further relates to polypeptides encoded by such a nucleic acid molecule, to constructs, vectors and cells comprising the nucleic acid molecule and to a use and method of using a polypeptide of the invention for degrading cellulose.
- Who is the assignee on this patent?
- Sintef Tto As, Sinteff Tto As
- What technology area does this patent fall under?
- Primary CPC classification C12N9/2437. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 02 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).