Methods for modulating the activity of insecticidal proteins

Patent metadata

Field	Value
Publication number	US-10934330-B2
Application number	US-201816208856-A
Country	US
Kind code	B2
Filing date	Dec 4, 2018
Priority date	Oct 2, 2009
Publication date	Mar 2, 2021
Grant date	Mar 2, 2021

Abstract

Compositions and methods for controlling pests are disclosed. In particular, methods for modulating the activity of Cry1Ba proteins against lepidopteran or coleopteran pests are provided. Further, mutant Cry1Ba proteins having modulated insecticidal activity compared to native Cry1Ba proteins are disclosed.

First claim

The invention claimed is:

1. A mutant Cry1Ba protein having toxicity to at least European corn borer ( Ostrinia nubilalis ) comprising a Trp (W) or a Phe (F) or a Pro (P) amino acid at an amino acid position corresponding to or at amino acid position 150 of SEQ ID NO: 2.

2. An insecticidal composition comprising the protein of claim 1 and an acceptable agricultural carrier.

3. The composition of claim 2 , wherein the agricultural carrier is a plant which expresses the protein.

4. A method of controlling at least European corn borer comprising contacting the European corn borer with the protein of claim 1 .

Assignees

• Syngenta Participations Ag

Inventors

• Gao Yan
• Conville Jared
• Chen Jeng Shong

Classifications

• C12N15/8286 Primary

  for insect resistance · CPC title

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• A01N47/44

  Guanidine; Derivatives thereof · CPC title

• A01H5/00

  Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title