DNA digital data storage device and method, and decoding method of DNA digital data

Patent metadata

Field	Value
Publication number	US-10929039-B2
Application number	US-201816138123-A
Country	US
Kind code	B2
Filing date	Sep 21, 2018
Priority date	Apr 11, 2018
Publication date	Feb 23, 2021
Grant date	Feb 23, 2021

Abstract

Disclosed is a storage method of DNA digital data, including: encoding a plurality of bit data to a plurality of base sequences including at least one degenerate base; and synthesizing at least two types of bases constituting the at least one degenerate base on a substrate based on a mixing ratio.

First claim

What is claimed is:

1. A generation method of DNA digital data, the method comprising:
encoding a plurality of bit data into a plurality of DNA base sequences including at least one degenerate base; and
synthesizing at least two types of bases constituting the at least one degenerate base based on a mixing ratio.

2. The generation method of claim 1 , further comprising:
synthesizing a single type of base among the plurality of base sequences.

3. The method of claim 1 , wherein:
the synthesizing of the at least one degenerate base includes,
incorporating the at least two types of bases based on the mixing ratio.

4. The generation method of claim 1 , wherein:
the synthesizing of the at least one degenerate base includes,
incorporating the at least two types of bases mixed according to the mixing ratio on the outside.

5. The generation method of claim 1 , wherein:
the ratios of the at least two types of bases are the same in the mixing ratio.

6. The generation method of claim 1 , wherein:
the ratios of the at least two types of bases are different in the mixing ratio.

7. The generation method of claim 1 , wherein:
the at least two types of bases are at least one of a DNA base, an RNA base, and a nucleic acid analogue.

8. Device for storing data in DNA in which a plurality of base sequences to which a plurality of bit data is encoded is divided and stored as a basic storage unit, the device comprising:
a plurality of storage units storing molecules in which a plurality of bases is synthesized based on a base sequence corresponding to the basic storage unit among the plurality of base sequences,
wherein the base sequence includes a first degenerate base consisting of at least two types of bases, and
the molecules include
first molecules in which a base is synthesized among the at least two types of bases, and
second molecules in which at least another base is synthesized among the at least two types of bases.

9. The storage device of claim 8 , wherein:
ratios between the first molecules and the second molecules follow a mixing ratio.

10. The storage device of claim 9 , wherein:
the ratios of the at least two types of bases are the same in the mixing ratio.

11. The storage device of claim 9 , wherein:
the ratios of the at least two types of bases are different in the mixing ratio.

12. The storage device of claim 8 , wherein:
the at least two types of bases are at least one of a DNA base, an RNA base, and a nucleic acid analogue.

13. A decoding method of DNA data, comprising:
categorizing a plurality of DNA fragments according to an address;
analyzing a base at the same position with respect to each of the plurality of categorized DNA fragments;
calculating a scatter plot of a base ratio based on a result of the analysis;
determining a base at a corresponding location as a degenerate base when the scatter plot of the base ratio is a mixing ratio of at least two bases; and
decoding data based on the determined degenerated base.

14. The decoding method of claim 13 , wherein:
the analyzing of the base includes,
analyzing a base call for each location of each of the plurality of DNA fragments.

15. The decoding method of claim 13 , wherein:
the base at the corresponding location is determined as a first degenerate base when the scatter plot of the base ratio is a first mixing ratio of at least two bases,
the base at the corresponding location is determined as a second degenerate base when the scatter plot of the base ratio is a second mixing ratio of the at least two bases, and
the first mixing ratio and the second mixing ratio are different from each other and the first degenerate base and the second degenerate base are different from each other.

Assignees

• Univ Industry Cooperation Group Kyung Hee Univ
• Seoul Nat Univ R&Db Foundation

Inventors

• Kwon Sunghoon
• Park Wook
• Choi Yeongjae
• Ryu Taehoon
• Song Suk-Heung
• Kim Hyeli
• Kim Seojoo

Classifications

• G16B30/00 Primary

  ICT specially adapted for sequence analysis involving nucleotides or amino acids · CPC title

• G06F3/0638 Primary

  Organizing or formatting or addressing of data · CPC title

• H03M7/30

  Compression (speech analysis-synthesis for redundancy reduction G10L19/00; for image communication H04N); Expansion; Suppression of unnecessary data, e.g. redundancy reduction · CPC title

• G06F3/0604

  Improving or facilitating administration, e.g. storage management · CPC title

• G06F3/0673

  Single storage device · CPC title