Activated platelet composition with tunable growth factor level

Patent metadata

Field	Value
Publication number	US-10927345-B2
Application number	US-201715694635-A
Country	US
Kind code	B2
Filing date	Sep 1, 2017
Priority date	Mar 31, 2015
Publication date	Feb 23, 2021
Grant date	Feb 23, 2021

Abstract

Methods and systems for generating a tunable or customizable activated product composition are related. In certain embodiments, one or more of electric pulse parameters, flow rate, or sample container size are varied so as to generate the activated product composition. The activated product composition may be customized or optimized based for a particular patient or procedure.

First claim

The invention claimed is:

1. A system, comprising:
a sample holder comprising electrodes, wherein the sample holder is configured to accept at least one cuvette;
pulse generating circuitry configured to generate one or more electrical pulses into a cuvette disposed in the sample holder;
processing circuitry configured:
to execute one or more processor-executable routines; and
to cause the pulse generating circuitry to generate the one or more electrical pulses; and

a non-transitory computer-readable memory storing the one or more processor-executable routines, wherein at least one of the one or more processor-executable routines, when executed, cause the processing circuitry to:
select a set of electrical pulse parameters from a plurality of sets of electrical pulse parameters stored in the non-transitory computer-readable memory, wherein the set of electrical pulse parameters is associated with a growth factor profile of a plurality of growth factor profiles, and the growth factor profile comprising an adjustably controlled level of at least one growth factor released by one or more of platelets, red blood cells, or white blood cells, and wherein the level of the at least one growth factor is substantially equal to or larger than a level of growth factor obtained with a thrombin activation;
configure the pulse generating circuitry to generate the one or more electrical pulses based on the set of electrical pulse parameters; and
cause the pulse generating circuitry to expose the cuvette disposed in the sample holder to the selected set of electrical pulses, to generate an activated product composition having the growth factor profile.

2. The system of claim 1 , wherein the at least one growth factor comprises platelet derived growth factor, epidermal growth factor, vascular endothelial growth factor, or transforming growth factor beta 1.

3. The system of claim 1 , wherein the sample holder is configured to accept a first cuvette type having a first electrode spacing dimension, and a second cuvette type having a second electrode spacing dimension, and wherein the one or more processor-executable routines, when executed, cause the processing circuitry to:
select the set of electrical pulse parameters from the plurality of sets of electrical pulse parameters based on the first electrode spacing dimension, the second electrode spacing dimension, or both.

4. The system of claim 1 , comprising a user input device configured to:
display a plurality of options, wherein each option is associated with a growth factor profile of the plurality of growth factor profiles; and
cause the processing circuitry to select a processor-executable routine of the one or more processor-executable routines stored in the non-transient memory based on a selected option.

5. The system of claim 1 , comprising current sensing circuitry configured to provide a sensed current across the electrodes of the sample holder to the processing circuitry, and wherein the at least one processor-executable routine of the one or more processor-executable routines is configured to update the set of electrical pulse parameters based on the sensed current.

6. The system of claim 1 , comprising a conduit coupled to the sample holder, wherein the conduit is configured to flow a sample into the sample holder and to flow the activated product composition from the sample holder, and wherein the at least one processor-executable routine of the one or more processor-executable routines is configured to select the set of electrical pulse parameters from the plurality of sets of electrical pulses based on a flow rate of the conduit.

7. The system of claim 1 , wherein the set of electrical pulse parameters comprise voltage, electric field, current, pulse width, energy density, energy per platelet, a number of pulses, or any combination thereof.

8. The system of claim 1 , wherein the one or more electrical pulses are configured to not cause lysis to platelets, red blood cells, or white blood cells.

9. An electric pulse generating system, comprising:
a sample holder comprising at least two electrodes;
pulse generating circuitry electrically coupled to the at least two electrodes of the sample holder;
a user input device; and
control circuitry configured to execute one or more processor-executable routines;
a non-transitory computer-readable memory storing the one or more processor-executable routines, wherein at least one of the one or more processor-executable routines, when executed, cause the control circuitry to:
receive instructions from the user input device;
generate one or more pulse parameters using a set of pulse parameters based on the received instruction, wherein the set of pulse parameters are stored in the non-transitory computer-readable memory; and
cause the pulse generating circuitry to provide a sequence of electrical pulses between the two electrodes based on the set of pulse parameters to transform a sample disposed in the sample holder into an activated product composition;

wherein the received instructions comprise a set of growth factors released by one or more platelets, red blood cells, or white blood cells and a plurality of growth factor levels, wherein each growth factor level is associated with one growth factor of the set of growth factors.

10. The electric pulse generating system of claim 9 , wherein at least one growth factor level is measured relative to a thrombin activation level.

11. The electric pulse generating system of claim 10 , wherein the at least one growth factor level is substantially higher than the thrombin activation level, substantially similar to the thrombin activation level, or substantially smaller than the thrombin activation level.

12. The electric pulse generating system of claim 10 , wherein the at least one growth factor level is a multiplier of the thrombin activation level.

13. The electric pulse generating system of claim 9 , wherein the set of growth factors comprise platelet derived growth factor, epidermal growth factor, vascular endothelial growth factor, or transforming growth factor beta 1.

14. The electric pulse generating system of claim 9 , wherein the sample holder is configurable to receive a cuvette or a conduit.

15. The electric pulse generating system of claim 14 , wherein the sequence of electric pulses is determined based on an electrode spacing of the cuvette or the conduit.

16. The electric pulse generating system of claim 9 , wherein the sample comprises a platelet rich plasma sample, a platelet suspension, or a whole blood sample.

17. The electric pulse generating system of claim 9 , wherein the set of pulse parameters comprise voltage, electric field, current, pulse width, energy density, energy per platelet, a number of pulses, or any combination thereof.

18. A system for customizing an activated blood-derived cell treatment comprising:
a sample holder configured to receive a sample comprising platelet rich plasma sample, a platelet suspension, or a whole blood sample;
pulse generating circuitry configured to expose the sample disposed in the sample holder to an electrical pulse sequence;
processing circuitry;
a user input device; and
a non-transient memory storing at least one processor-executable routine that when executed causes the processing circuitry to:
receive a selection for treating a patient, wherein the selection comprises a type of wound, a wound healing cascade process, or both;
generate a customized growth factor profile based on the selection for treating the patient by adjustably contr

Assignees

• Gen Electric

Inventors

• Neculaes Vasile Bogdan
• Becerra Juan Jose
• Morton Christine Angela
• Torres Andrew Soliz
• Caiafa Antonio

Classifications

• C12N2529/00

  Culture process characterised by the use of electromagnetic stimulation · CPC title

• C12M1/36

  including condition or time responsive control, e.g. automatically controlled fermentors (controlling or regulating in general G05) · CPC title

• C12N5/0644 Primary

  Platelets; Megakaryocytes · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M1/42

  Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title