Serum albumin-binding fibronectin type III domains

Patent metadata

Field	Value
Publication number	US-10925932-B2
Application number	US-201715611296-A
Country	US
Kind code	B2
Filing date	Jun 1, 2017
Priority date	Jun 3, 2016
Publication date	Feb 23, 2021
Grant date	Feb 23, 2021

Abstract

Fibronectin type III domains (FN3) that specifically bind to serum albumin, related polynucleotides capable of encoding serum albumin-specific FN3 domains, cells expressing the FN3 domains, as well as associated vectors, detectably labeled FN3 domains and FN3 domains fused to a heterologous moiety are useful in extending the half-life of molecules in diagnostic and therapeutic applications.

First claim

We claim:

1. A protein comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 51 or an amino acid sequence having 1, 2, 3, 4, 5, 6, 7, or 8 substitutions as compared to the amino acid sequence of SEQ ID NO: 51.

2. The protein of claim 1 , wherein the protein comprises an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 51.

3. The protein of claim 1 , wherein the protein is at least 95%, 97%, 98%, or 99% identical to the amino acid sequence of SEQ ID NO: 51.

4. The protein of claim 1 , wherein the protein comprises an amino acid sequence that has 1, 2, 3, 4, 5, 6, 7, or 8 substitutions when compared to the amino acid sequence of SEQ ID NO: 51.

5. A pharmaceutical composition comprising the protein of claim 1 and a pharmaceutically acceptable carrier.

6. A kit comprising the protein of claim 1 .

7. A molecule comprising a first domain and a second domain, wherein the first domain comprises the protein of claim 1 and the second domain is a molecule that binds to a target protein other than human serum albumin.

8. The protein of claim 1 , wherein the protein is at least 95% identical to the amino acid sequence of SEQ ID NO: 51.

9. The protein of claim 1 , wherein the protein is at least 97% identical to the amino acid sequence of SEQ ID NO: 51.

10. The protein of claim 1 , wherein the protein is at least 98% identical to the amino acid sequence of SEQ ID NO: 51.

11. The protein of claim 1 , wherein the protein is at least 99% identical to the amino acid sequence of SEQ ID NO: 51.

12. The protein of claim 1 , wherein the protein comprises the amino acid sequence of SEQ ID NO: 51.

13. A method of detecting the presence of human serum albumin in a biological sample comprising contacting the biological sample with the protein of claim 1 and determining the binding of the biological sample to the protein.

14. A protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 51-53.

15. A protein comprising the amino acid sequence of SEQ ID NO: 52.

16. A protein comprising the amino acid sequence of SEQ ID NO: 53.

Assignees

• Janssen Biotech Inc

Inventors

• Diem Michael
• Jacobs Steven
• O'Neil Karyn
• Rutkoski Thomas

Classifications

• G01N2333/765

  Serum albumin, e.g. HSA · CPC title

• G01N33/6803

  General methods of protein analysis not limited to specific proteins or families of proteins · CPC title

• C07K2317/31

  multispecific · CPC title

• C07K2317/94

  Stability, e.g. half-life, pH, temperature or enzyme-resistance · CPC title

• C07K2319/30

  Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto · CPC title