Device for real-time measurement of bacteria by atp detection

Patent metadata

Field	Value
Publication number	US-10920266-B2
Application number	US-201816221585-A
Country	US
Kind code	B2
Filing date	Dec 17, 2018
Priority date	Jan 3, 2018
Publication date	Feb 16, 2021
Grant date	Feb 16, 2021

Abstract

Provided is a device for real-time measurement of bacteria. The device for real-time measurement of bacteria includes reaction portions, a support portion configured to support the reaction portions, a rotational shaft configured to transfer the support portion, and a sample supply portion configured to supply a sample to each of the reaction portions, and according to the device for real-time measurement of bacteria, bacteria may be measured in real time through the detection of ATP.

First claim

What is claimed is:

1. A device for real-time measurement of bacteria, the device comprising:
at least two reaction portions, each having a reagent for adenosine triphosphate (ATP) detection immobilized thereon;
a flexible support portion configured to support the at least two reaction portions;
a rotational shaft connected to the support portion and configured to move the support portion; and
a sample supply portion configured to supply a sample to each of the reaction portions,
wherein the at least two reaction portions comprise a reagent for ATP detection comprising luciferase and luciferin and a member configured to dry and immobilize the reagent, and
wherein the at least two reaction portions are arranged on the support portion at regular intervals.

2. The device of claim 1 , wherein the reagent further comprises at least one of dithiothreitol (DTT), Tris, glycine, MgSO 4 , ethylenediaminetetraacetic acid (EDTA), or sodium azide.

3. The device of claim 1 , wherein the support portion is formed of a non-moisture permeable material.

4. The device of claim 1 , wherein the sample supply portion comprises a bacteria collector configured to collect bacteria and a bacteria lysis portion configured to lyse bacteria.

5. The device of claim 4 , wherein the bacteria collector collects airborne bacteria by exposing a solution to the air.

6. The device of claim 4 , wherein the bacteria lysis portion performs heat treatment on the collected bacteria.

7. The device of claim 1 , wherein the member configured to dry and immobilize the reagent is at least one of paper, natural fiber, synthetic fiber, or porous material.

8. The device of claim 1 , wherein the support portion and the at least two reaction portions are capable of being wound in a cylindrical form according to rotation of the rotational shaft.

9. The device of claim 1 , wherein the support portion and the at least two reaction portions are detachable from the device for real-time measurement of bacteria.

10. The device of claim 1 , further comprising a detector configured to detect a reaction occurring in each of the reaction portions.

11. The device of claim 10 , wherein the detector is a luminometer.

12. A method of measuring bacteria in real time by using the device of claim 1 , the method comprising:
supplying a sample from a sample supply portion to a first reaction portion;
moving a support portion using a rotational shaft, thereby placing a second reaction portion at a position where the second reaction portion is able to receive the sample from the sample supply portion;
detecting ATP present in the first reaction portion; and
repeating the above processes at least once by using at least two reaction portions.

Assignees

• Korea Inst Sci & Tech

Inventors

• Kim Byoung Chan
• Jung Jae Hee
• Kim Hye Ri
• Cho Yusung
• Lee Kang Bong

Classifications

• G01N21/77

  by observing the effect on a chemical indicator · CPC title

• C12Q2304/60

  Chemiluminescent detection using ATP-luciferin-luciferase system · CPC title

• C12Q1/66 Primary

  involving luciferase · CPC title

• C12Q1/04

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• C12Q1/008 Primary

  for determining co-enzymes or co-factors, e.g. NAD, ATP · CPC title