Enzymatic method for producing 2-hydroxy-4-methylmercaptobutanoic acid (MHA)
US-10815508-B2 · Oct 27, 2020 · US
Process for producing L-methionine from methional
US10920255B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10920255-B2 |
| Application number | US-201716466337-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 30, 2017 |
| Priority date | Dec 5, 2016 |
| Publication date | Feb 16, 2021 |
| Grant date | Feb 16, 2021 |
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Abstract
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A method is useful for the biocatalytic synthesis of proteinogenic L-amino acids, such as L-alanine, L-valine, L-methionine, L-leucine, L-isoleucine or L-phenylalanine from a respective aldehyde and carbon dioxide. In particular, the method is useful for the biocatalytic synthesis of L-methionine from 3-methylthio-propanal (“methional”) and carbon dioxide.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for producing an L-amino acid, comprising: reacting a mixture comprising an aldehyde, carbon dioxide, a decarboxylase, a corresponding decarboxylase cofactor, and (a) at least one donor amino acid and an aminotransferase, and/or (b) NADH, ammonia and/or an ammonium salt and an amino acid dehydrogenase, thereby producing said L-amino acid or a salt thereof, wherein the L-amino acid produced is L-methionine and the aldehyde is 3-(methylthio)-propanal (methional), wherein the decarboxylase is at least one member selected from the group consisting of pyruvate decarboxylase PDC1, which originates from Saccharomyces cerevisiae , phenylpyruvate decarboxylase ARO10, which originates from Saccharomyces cerevisiae , and branched chain decarboxylase KdcA, which originates from Lactococcus lactis, wherein the aminotransferase, if present, is at least one member selected from the group consisting of methionine aminotransferase YbdL, which originates from E. coli , and aromatic aminotransferase Aro8, which originates from Saccharomyces cerevisiae , and wherein the amino acid dehydrogenase, if present, is at least one member selected from the group consisting of leucine dehydrogenase (LeuDH), which originates from Bacillus sphaericus , and phenylalanine dehydrogenase (PheDH), which originates from Thermoactinomyces intermedius. 2. The method as claimed in claim 1 , wherein the corresponding decarboxylase cofactor comprises thiamine pyrophosphate. 3. The method as claimed in claim 1 , wherein the donor amino acid is present, is different from the L-amino acid produced, and is at least one L-amino acid selected from the group consisting of L-glutamine, L-glutamate, L-alanine, L-phenylalanine, L-tyrosine, L-leucine, L-isoleucine, L-histidine and L-tryptophan. 4. The method as claimed in claim 1 , wherein the carbon dioxide is applied to the mixture at a pressure from 10 to 7400 kPa. 5. The method as claimed in claim 1 , wherein the mixture further comprises formic acid or a salt thereof and a formate dehydrogenase. 6. The method as claimed in claim 5 , wherein the formate dehydrogenase is at least one member selected from the group consisting of formate dehydrogenase from Pseudomonas sp. and formate dehydrogenase from Candida sp. as well as mutants and variants of these formate dehydrogenases having formate dehydrogenase activity.
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Classifications
Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine · CPC title
- C12P13/12Primary
Methionine; Cysteine; Cystine · CPC title
Lysine; Diaminopimelic acid; Threonine; Valine · CPC title
- C12P13/06Primary
Alanine; Leucine; Isoleucine; Serine; Homoserine · CPC title
Phenylalanine dehydrogenase (1.4.1.20) · CPC title
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- What does patent US10920255B2 cover?
- A method is useful for the biocatalytic synthesis of proteinogenic L-amino acids, such as L-alanine, L-valine, L-methionine, L-leucine, L-isoleucine or L-phenylalanine from a respective aldehyde and carbon dioxide. In particular, the method is useful for the biocatalytic synthesis of L-methionine from 3-methylthio-propanal (“methional”) and carbon dioxide.
- Who is the assignee on this patent?
- Evonik Operations Gmbh
- What technology area does this patent fall under?
- Primary CPC classification C12P13/12. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 16 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 6 related publications on this page (citations in our corpus or others sharing the same primary CPC).