Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
US-10619204-B2 · Apr 14, 2020 · US
Flow cells with hydrogel coating
US10919033B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10919033-B2 |
| Application number | US-201816626393-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 17, 2018 |
| Priority date | Dec 21, 2017 |
| Publication date | Feb 16, 2021 |
| Grant date | Feb 16, 2021 |
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- Title
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Abstract
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In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.
First claim
Opening claim text (preview).
What is claimed is: 1. A flow cell, comprising: a patterned substrate including depressions separated by interstitial regions and including a bonding region; sequencing surface chemistry attached to each of the depressions, the sequencing surface chemistry including: a functionalized coating layer; and a primer grafted to the functional coating layer; a non-grafted hydrogel on at least the sequencing surface chemistry, the non-grafted hydrogel selected from the group consisting of crosslinked polyacrylamide, an agarose gel, and crosslinked polyethylene glycol; a lid attached to the patterned substrate at the bonding region; and a flow channel defined between the patterned substrate and the lid. 2. The flow cell as defined in claim 1 , wherein the non-grafted hydrogel is also on at least some of the interstitial regions. 3. The flow cell as defined in claim 1 , wherein the functionalized coating layer is poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide). 4. The flow cell as defined in claim 1 , further comprising a spacer layer bonding the lid to the bonding region. 5. The flow cell as defined in claim 1 , wherein the non-grafted hydrogel is the crosslinked polyethylene glycol, and wherein the crosslinked polyethylene glycol includes a polyethylene glycol macromer crosslinked with a reactive chain end selected from the group consisting of acrylate, methacrylate, allyl ether, maleimide, vinyl sulfone, N-hydroxysuccinimide ester, and vinyl ether.
Assignees
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Classifications
fluorescence · CPC title
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes · CPC title
- C12Q1/6874Primary
involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title
Polyalkylene oxides · CPC title
Homopolymers or copolymers of acrylamide or methacrylamide · CPC title
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- What does patent US10919033B2 cover?
- In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.
- Who is the assignee on this patent?
- Illumina Inc, Illumina Cambridge Ltd
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6874. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 16 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).