Device for DNA sample fragmentation configured to produce ultrasonic waves

Patent metadata

Field	Value
Publication number	US-10919018-B2
Application number	US-201716094798-A
Country	US
Kind code	B2
Filing date	Jun 19, 2017
Priority date	Apr 19, 2016
Publication date	Feb 16, 2021
Grant date	Feb 16, 2021

Abstract

A device for the fragmentation of DNA samples. The DNA samples are in solution in a container the device including —a vessel for receiving a liquid, the vessel being configured to produce ultrasonic waves so as to spread ultrasonic waves through the liquid. The device further includes a first support element resting on the vessel, the device further includes a second support element having a passage designed to receive the container. The second support element can be suspended by at least one suspension element forming at least one joint, such that a lower portion of the container can be immersed in the liquid.

First claim

The invention claimed is:

1. A device for the fragmentation of DNA samples that are in solution in a container by presenting a substantially homogenous field of ultrasonic waves in the vicinity of the container, the device comprising:
a vessel for receiving a liquid, the vessel presenting a lateral face and being provided with means for producing ultrasonic waves so as to spread ultrasonic waves through the liquid;
an outer frame at least partially disposed around at least a portion of the vessel so as to insulate ultrasonic waves propagating within the vessel from an outside environment; and
a first support element resting on the vessel,

the device further comprising a second support element comprising a passage designed to receive the container, the second support element being suspended from the first support element at a distance from said first support element by at least one suspension element forming at least one joint operably coupling the first support element and the second support element in three degrees of freedom to allow for the immersion of a lower portion of the container in the liquid, the vessel including structure defining an overflow device positioned along the lateral face of the vessel for precisely controlling the level of liquid received by the vessel, whereby the distance that the second support element is suspended from the first support element and the position of the overflow device along the lateral face of the vessel, provide for precise positioning of the container with respect to the level of liquid received by the vessel;
wherein the overflow device includes a conduit extending between the vessel and the outer frame and to an exterior portion of the outer frame so as to convey excess liquid outside of the outer frame.

2. The device according to claim 1 wherein the at least one suspension element is deformable.

3. The device according to claim 1 wherein the at least one suspension element comprises a means for adjusting arranged to modify the distance between the first support element and the second support element.

4. The device according to claim 1 wherein the means for producing ultrasonic waves comprises at least one ultrasonic transducer.

5. The device according to claim 4 wherein the means for producing ultrasonic waves further comprises a signal generator arranged to supply the at least one ultrasonic transducer with an electric signal.

6. The device according to claim 5 wherein the signal generator comprises a module for generating periodic signals.

7. The device according to claim 1 wherein the means for producing ultrasonic waves is fastened onto an outer lower face of the vessel.

8. The device according to claim 7 wherein the means for producing ultrasonic waves is placed substantially vertically with the passage of the second support element.

9. The device according to claim 1 wherein the first support element comprises a passage in such a way as to allow a user to access the passage of the second support element.

10. The device according to claim 1 wherein the second support element is separated from the lateral face of the vessel by a predetermined distance in such a way as to limit the exposure of the container to edge effects in the vicinity of the lateral face created by the reflection of the ultrasonic waves by the lateral face.

Assignees

• Francais Du Sang Ets
• Etablissement Franais Du Sang

Inventors

• Alizadeh Medhi
• Mahdjoubi Cyrus

Classifications

• G01N1/28

  Preparing specimens for investigation {including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q}(mounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 {; laboratory gas handling apparatus B01L5/00}) · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• B06B3/00

  Methods or apparatus specially adapted for transmitting mechanical vibrations of infrasonic, sonic, or ultrasonic frequency · CPC title

• B01F31/87 Primary

  transmitting the vibratory energy by means of a fluid, e.g. by means of air shock waves · CPC title

• B01J19/10 Primary

  employing sonic or ultrasonic vibrations · CPC title