Plant transformation without selection
US-2019218562-A1 · Jul 18, 2019 · US
Method and apparatus for substantially isolating plant tissues
US10918032B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10918032-B2 |
| Application number | US-201816106342-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 21, 2018 |
| Priority date | Aug 31, 2007 |
| Publication date | Feb 16, 2021 |
| Grant date | Feb 16, 2021 |
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Abstract
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The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
First claim
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What is claimed is: 1. An apparatus for preparing plant embryo tissue suitable for tissue culture and/or genetic transformation comprising (a) a container for holding plant seed tissue comprising a plurality of plant embryos in an aqueous environment, wherein the container comprises plant embryo tissue; and (b) at least a first nozzle for delivering to the aqueous environment an agent that selectively attaches to the embryos, wherein the nozzle produces gas bubbles with an average diameter of from about 100 microns to about 1 mm. 2. The apparatus of claim 1 , wherein the container further comprises media and non-embryo tissue. 3. The apparatus of claim 1 , further comprising a collector for separating embryo tissue based on the buoyancy of the embryos within the aqueous environment. 4. The apparatus of claim 1 , wherein the gas bubbles comprises a gas selected from the group consisting of air, oxygen, nitrogen, and a combination thereof. 5. The apparatus of claim 1 , wherein the embryo tissue is comprised within a mixture of embryos and endosperm. 6. The apparatus of claim 1 , wherein the nozzle comprises a polar surface. 7. The apparatus of claim 1 , wherein the nozzle comprises glass, limewood, or ceramic. 8. The apparatus of claim 1 , comprising multiple point sources of bubbles. 9. The apparatus of claim 3 , wherein the embryo tissue is collected by skimming a froth comprising bubbles and embryos. 10. The apparatus of claim 3 , wherein the embryo tissue is collected by application of vacuum. 11. The apparatus of claim 1 , wherein the aqueous environment comprises a surfactant. 12. The apparatus of claim 11 , wherein the surfactant is selected from the group consisting of: a polyether, polypropylene glycol, and polyethylene glycol. 13. The apparatus of claim 1 , wherein the plant embryo tissue is maize embryo tissue, cotton embryo tissue, or soybean embryo tissue.
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Frequently asked questions
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- What does patent US10918032B2 cover?
- The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
- Who is the assignee on this patent?
- Monsanto Technology Llc
- What technology area does this patent fall under?
- Primary CPC classification A01H4/001. Mapped technology areas include Human Necessities.
- When was this patent published?
- Publication date Tue Feb 16 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 6 related publications on this page (citations in our corpus or others sharing the same primary CPC).