Culture medium additive, culture medium composition, and method for culturing cells or tissue using same

Patent metadata

Field	Value
Publication number	US-10914725-B2
Application number	US-201615566981-A
Country	US
Kind code	B2
Filing date	Apr 15, 2016
Priority date	Apr 16, 2015
Publication date	Feb 9, 2021
Grant date	Feb 9, 2021

Abstract

The invention provides a medium additive, medium composition and a culture method and the like, capable of efficiently culturing cells or tissues in a well dispersed state, and further, permitting cell image analysis of the cells or tissues. The medium additive or medium composition contains agar, which preferably is a low molecular weight agar having a weight average molecular weight of 10,000-60,000. Using same, cells or tissues can be cultured in a well-dispersed state in a medium, and a proliferation promoting effect for the cells or tissues can also be obtained. In addition, the cells can be cultured in any of a floating state and a precipitated state by adjusting the concentration of the aforementioned agar.

First claim

The invention claimed is:

1. A method of culturing a cell or tissue, comprising cultivating the cell or tissue in a precipitated state on the bottom of a culture container but in a dispersed state in a medium composition comprising agar having a weight average molecular weight of 10,000-60,000,
wherein the agar is present in the medium composition in an amount of not less than 0.005 (w/v)% and less than 0.07 (w/v)% relative to the total amount of the medium composition, and
wherein the medium composition has a viscosity at 37° C. of not more than 2.5 mPa·s when the agar content is 0.1 (w/v)%.

2. The method according to claim 1 , wherein the cell is an adherent cell or a floating cell.

3. The method according to claim 2 , wherein the adherent cell is adhered to a carrier surface or embedded inside a carrier.

4. The method according to claim 2 , wherein the adherent cell is adhered to a microcarrier.

5. The method according to claim 2 , wherein the adherent cell forms a sphere.

6. The method according to claim 2 , wherein the adherent cell is selected from the group consisting of a cancer cell, a hepatocyte and a cancer cell line.

Assignees

• Nissan Chemical Ind Ltd

Inventors

• Abe Natsuki
• Nishino Taito
• Otani Ayako

Classifications

• G01N33/5067

  Liver cells · CPC title

• G01N33/5011 Primary

  for testing antineoplastic activity · CPC title

• C08L89/00

  Compositions of proteins; Compositions of derivatives thereof (foodstuff preparations A23J3/00) · CPC title

• C08L5/12 Primary

  {Agar or} agar-agar {, i.e. mixture of agarose and agaropectin}; Derivatives thereof · CPC title

• G01N33/5044

  involving specific cell types · CPC title