Modular platform for tissue integrated cell culture

Patent metadata

Field	Value
Publication number	US-10913925-B2
Application number	US-201615360457-A
Country	US
Kind code	B2
Filing date	Nov 23, 2016
Priority date	Jul 25, 2012
Publication date	Feb 9, 2021
Grant date	Feb 9, 2021

Abstract

The systems and methods disclosed herein are generally related to a cell culture system. More particularly, the systems and methods enable the culturing and interconnecting of a plurality of tissue types in a biomimetic environment. By culturing organ specific tissue types within a biomimetic environment and interconnecting each of the organ systems in a physiologically meaningful way, experiments can be conducted on in vitro cells that substantially mimic the responses of in vivo cell populations. In some implementations, the system is used to monitor how organ systems respond to agents such as toxins or medications. The system enables the precise and controlled delivery of these agents, which, in some implementations, enables the biomimetic dosing of drugs in humans to be mimicked.

First claim

What is claimed:

1. A system for culturing cells, comprising:
a disposable fluid routing plate including:
a planar upper mounting surface; a plurality of fluid channels; a plurality of locking and alignment recesses and flow ports extending within the planar upper mounting surface, each flow port being positioned relative to a separate corresponding subset of the locking and alignment recesses, the locking and alignment recesses configured for removably accepting mating locking and alignment features associated with respective individual first and second cell culture vessels for separately removably mounting the first and second cell culture vessels with mating surfaces on top of the planar upper mounting surface of the fluid routing plate at selected locations associated with selected flow ports, and properly aligning the first and second cell culture vessels with the selected flow ports, and for coupling the first and second cell culture vessels to selected respective channels of the plurality of fluid channels via the selected flow ports; and at least one valve coupled to at least two of the fluid channels configured to control a path of a fluid flow through the plurality of fluid channels to form a fluid path for respective first and second cell culture vessels; and
a reusable control plate configured for coupling to the fluid routing plate, including an actuator for controlling the at least one valve in the fluid routing plate.

2. The system of claim 1 , further comprising the first and second cell culture vessels.

3. The system of claim 1 , further comprising a processor coupled to the control plate and configured to output control signals to the control plate to configure the position of the actuator.

4. The system of claim 1 , further comprising a sensor for measuring a parameter of a cell culture disposed in one of the first and second cell culture vessels.

5. The system of claim 4 , further comprising a computer processor coupled to the sensor configured to receive data from the sensor to monitor the measured cell culture parameter.

6. The system of claim 1 , wherein at least one of the receptacles is configured to receive a plurality of types of modular cell culture vessels configured for culturing different respective cell types.

7. The system of claim 1 , further comprising a second actuator configured to introduce a predetermined amount of an agent into the plurality of fluid channels.

8. The system of claim 7 , wherein the agent is one of a medication and a toxin.

9. The system of claim 1 , further comprising a second actuator configured to withdraw a fluid sample from the plurality of fluid flow channels.

10. The system of claim 1 , wherein the actuator is configured to control the state of the valve within the fluid routing plate to control flow rates of the fluid flow through the fluid routing plate.

11. The system of claim 10 , wherein the actuator is configured to close the valve by deforming a control membrane within the valve, such that the membrane substantially impedes the flow of fluid through a channel in the fluid routing plate.

12. The system of claim 1 , further comprising a fluid pump coupled to one of the control plate and the fluid routing plate to generate a fluid flow along the fluid path for respective first and second cell culture vessels.

13. The system of claim 1 in which the plurality of locking and alignment recesses and flow ports are of a quantity capable of accepting more than two cell culture vessels.

14. The system of claim 13 in which the locking and alignment recesses are mortises for engaging mating tenons.

15. The system of claim 13 in which more than 20 cell culture vessels are capable of being mounted on the planar upper mounting surface.

Assignees

• Charles Stark Draper Laboratory Inc
• The Massachusetts Institute Of Tech

Inventors

• Cuiffi Joseph
• Borenstein Jeffrey T
• Achyuta Anilkumar Harapanahalli
• Mescher Mark J
• Griffith Linda
• Inman Samuel Walker

Classifications

• C12M23/42

  Integrated assemblies, e.g. cassettes or cartridges · CPC title

• C12M23/58

  Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00) · CPC title

• C12M41/00

  Means for regulation, monitoring, measurement or control, e.g. flow regulation (controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N) · CPC title

• C12M23/44 Primary

  Multiple separable units; Modules · CPC title

• C12M23/12

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title