Fluorescence-based sensor with multiple indicator grafts

Patent metadata

Field	Value
Publication number	US-10912498-B2
Application number	US-201816132615-A
Country	US
Kind code	B2
Filing date	Sep 17, 2018
Priority date	Nov 15, 2013
Publication date	Feb 9, 2021
Grant date	Feb 9, 2021

Abstract

A system including a sensor and a transceiver and configured to determine a concentration of an analyte in a medium of a living animal. The sensor may include first and second signal photodetectors and first and second reference photodetectors, which may each be covered by an associate filter. The first photodetector may receive light emitted from first and second grafts, respectively. The grafts may receive excitation light from one or more light sources. The transceiver may receive signals from the photodetectors of the sensor and may determine the analyte concentration based on the received signals.

First claim

What is claimed is:

1. A system for determining a concentration of an analyte in a medium of a living animal, the system comprising:
an optical sensor, the sensor including:
a sensor housing;
a first graft covering at least a first portion of the sensor housing;
first indicator molecules embedded in the first graft and having a first optical characteristic responsive to the concentration of the analyte;
a second graft covering at least a second portion of the sensor housing;
second indicator molecules embedded in the second graft and having a second optical characteristic responsive to the concentration of the analyte;
a substrate;
a first light source fabricated in or mounted on the substrate and configured to emit first excitation light to the first indicator molecules in the first graft;
a first signal photodetector fabricated in or mounted on the substrate and configured to receive first emission light emitted by the first indicator molecules and output a signal indicative thereof;
a second light source fabricated in or mounted on the substrate and configured to emit second excitation light to the second indicator molecules in the second graft; and
a second signal photodetector fabricated in or mounted on the substrate and configured to receive second emission light emitted by the second indicator molecules and output a signal indicative thereof; and

a transceiver configured to:
receive the signals indicative of the first signal light and the second signal light;
determine whether the first graft is damaged or degraded using one or more of the received signals; and
if the first graft is determined to be damaged or degraded, calculate a concentration of the analyte in the medium of the living animal based on the received signal indicative of the second signal light but not the received signal indicative of the first signal light.

2. The system of claim 1 , wherein the sensor further comprises:
a first signal filter configured to pass only a first narrow band of wavelengths including the wavelength of the first emission light emitted by the first indicator molecules, wherein the first signal filter covers a light receiving surface of the first signal photodetector; and
a second signal filter configured to pass only a second narrow band of wavelengths including the wavelength of the second emission light emitted by the second indicator molecules, wherein the second signal filter covers a light receiving surface of the second signal photodetector.

3. The system of claim 2 , wherein the wavelength of the first emission light emitted by the first indicator molecules is different than the wavelength of the second emission light emitted by the second indicator molecules.

4. The system of claim 1 , wherein the first indicator molecules are different than the second indicator molecules, and the wavelength of the first emission light emitted by the first indicator molecules is different than the wavelength of the second emission light emitted by the second indicator molecules.

5. The system of claim 1 , wherein the first indicator molecules are identical to the second indicator molecules, and the wavelength of the first emission light emitted by the first indicator molecules is the same as the wavelength of the second emission light emitted by the second indicator molecules.

6. The system of claim 1 , wherein the sensor further comprises an inductive element configured to convey signals generated by the first signal photodetector and the second signal photodetector.

7. The system of claim 1 , wherein the sensor further comprises a third signal photodetector fabricated in or mounted on the substrate and configured to receive third emission light.

8. The system of claim 7 , wherein the sensor further comprises third indicator molecules embedded in the first graft and having a third optical characteristic responsive to the concentration of the analyte, and the third emission light is emitted by third indicator molecules embedded in the first graft.

9. The system of claim 7 , wherein the sensor further comprises:
a third graft covering at least a third portion of the sensor housing; and
third indicator molecules embedded in the third graft and having a third optical characteristic responsive to the concentration of the analyte;
wherein the third emission light is emitted by the third indicator molecules.

10. The system of claim 1 , wherein the sensor further comprises a temperature sensor configured to output a temperature signal indicative of the temperature of the sensor, and the transceiver is further configured to receive the temperature signal and use the received temperature signal in the calculation of the analyte concentration.

11. The system of claim 1 , wherein the sensor further comprises first and second temperature sensors configured to output first and second temperature signals, respectively, and the transceiver is further configured to receive the first and second temperature signals and use the received first and second temperature signals in the calculation of the analyte concentration.

12. The system of claim 1 , wherein the transceiver is further configured to:
determine whether the second graft is damaged or degraded using one or more of the received signals; and
if the second graft is determined to be damaged or degraded, calculate a concentration of the analyte in the medium based on the received signal indicative of the first signal light but not the received signal indicative of the second signal light.

13. The system of claim 1 , wherein:
the sensor housing has a first side and a second side opposite the first side;
the first portion is a portion of the first side of the sensor housing;
the second portion is a portion of the second side of the sensor housing;
the substrate has a first side and a second side opposite the first side of the substrate;
the first light source is fabricated in or mounted on the first side of the substrate;
the first signal photodetector is fabricated in or mounted on the first side of the substrate;
the second light source is fabricated in or mounted on the second side of the substrate; and
the second signal photodetector is fabricated in or mounted on the second side of the substrate.

14. The system of claim 1 , wherein:
the sensor housing has a first side and a second side opposite the first side;
the second portion is a portion of the second side of the sensor housing;
the substrate has a first side and a second side opposite the first side of the substrate; and
the first light source, the first signal photodetector, the first reference photodetector, the second light source, the second signal photodetector, and the second reference photodetector are fabricated in or mounted on the first side of the substrate.

15. The system of claim 1 , wherein the wavelength of the first excitation light emitted by the first light source is different than the wavelength of the second excitation light emitted by the second light source.

16. The system of claim 1 , wherein the transceiver comprises an inductive element.

17. A method for determining a concentration of an analyte in a medium of a living animal, the method comprising:
using a first signal photodetector to receive first emission light emitted by first indicator molecules of a first graft covering at least a first portion of a sensor housing of an optical sensor, wherein the first indicator molecules have a first optical characteristic responsive to the concentration of the analyte in the medium;
using the first signal photodetector to ou

Assignees

• Senseonics Inc

Inventors

• Emken Jeremy
• Huffstetler Philip
• Whitehurst Todd

Classifications

• A61B5/1451 Primary

  for interstitial fluid · CPC title

• A61B5/0031

  Implanted circuitry · CPC title

• A61B5/1473

  invasive, e.g. introduced into the body by a catheter · CPC title

• A61B2560/0219

  of externally powered implanted units · CPC title

• A61B5/14532

  for measuring glucose, e.g. by tissue impedance measurement · CPC title