Optical density system and related method thereof

Patent metadata

Field	Value
Publication number	US-10907122-B2
Application number	US-201615569195-A
Country	US
Kind code	B2
Filing date	Apr 29, 2016
Priority date	Apr 29, 2015
Publication date	Feb 2, 2021
Grant date	Feb 2, 2021

Abstract

An apparatus and related method for determining the optical density and/or change in optical density of a reaction mixture in an agitated vessel or container. The apparatus may include at least one electromagnetic emitter configured to pass radiation through the reaction mixture to at least one receptor, whereby the receptor receives the transmitted or scattered light, while the reaction mixture is subjected to shaking. The emitters may have an interchangeable association with the vessel or container and/or the receptors may have an interchangeable association with the vessel or container. Further, a processor may be provided to receive the transmitted or scattered light data and filter to eliminate or reduce the effect of the frequency at which the reaction mixture is shaken. The processor analyzes the filtered data to provide the optical density and/or change in optical density of the reaction mixture.

First claim

We claim:

1. A method for determining the optical density and/or change in optical density of a reaction mixture in an agitated vessel or container, said method comprising:
passing radiation through the mixture from at least one electromagnetic emitter to at least one receptor;
receiving the radiation passed through the reaction mixture with said at least one receptor;
measuring the transmitted or scattered light detected by said at least one receptor;
shaking said vessel or container and said reaction mixture during said measurement of the transmitted or scattered light;
individually passing the raw measurement from each of said at least one receptors through a frequency filter of predetermined width to eliminate or reduce the effect of the frequency of the shaken reaction mixture; and
analyzing the filtered data to provide the optical density and/or change in optical density of said reaction mixture.

2. The method of claim 1 , wherein the detection of emitted radiation is effected at a frequency that is substantially greater than the frequency at which said vessel or container is shaken.

3. The method of claim 1 , wherein said frequency filter of predetermined width is a Gaussian moving average window filter.

4. The method of claim 3 , wherein the Gaussian moving average window length is approximately 5.6 seconds.

5. The method of claim 3 , wherein the Gaussian moving average window length is approximately five periods at the lowest shaking speed of the vessel or container.

6. The method of claim 3 , wherein the Gaussian moving average window length allows for approximately 800 measurements.

7. The method of claim 1 , wherein said at least one emitter is configured to be in interchangeable association with said vessel or container.

8. The method of claim 1 , wherein said at least one receptor is configured to be in interchangeable association with said vessel or container.

9. The method of claim 1 , wherein passing radiation comprises positioning said at least one receptor externally to the bottom of said vessel or container wherein the vessel or container is substantially transparent to the radiation emitted by said at least one emitter.

10. The method of claim 9 , wherein said at least one receptor is positioned proximal to or at the perimeter of the bottom of said vessel or container.

11. The method of claim 1 , further comprising comparing said individual filtered applications to determine the eliminated or reduced effect for each of said at least one receptors.

12. An apparatus for determining the optical density and/or change in optical density of a reaction mixture in an agitated vessel or container, said apparatus comprising:
at least one electromagnetic emitter configured to pass radiation through the reaction mixture to at least one receptor, said at least one receptor configured to receive the transmitted or scattered light, while the reaction mixture is subjected to shaking;
a processor configured to receive the transmitted or scattered light data, wherein said received data from each of said at least one receptor is individually passed through a frequency filter of predetermined width to eliminate or reduce the effect of the frequency at which the reaction mixture is shaken; and
said processor configured to analyze the filtered data to provide the optical density and/or change in optical density of said reaction mixture.

13. The apparatus of claim 12 , wherein the detection of emitted radiation is effected at a frequency that is substantially greater than the frequency of shaking at which said vessel or container is subjected.

14. The apparatus of claim 12 , wherein said frequency filter of predetermined width is a Gaussian moving average window filter.

15. The apparatus of claim 14 , wherein the Gaussian moving average window length is approximately 5.6 seconds.

16. The apparatus of claim 14 , wherein the Gaussian moving average window length is approximately five periods at the lowest shaking speed of the vessel or container.

17. The apparatus of claim 14 , wherein the Gaussian moving average window length allows for approximately 800 measurements.

18. The apparatus of claim 12 , wherein said at least one emitter is configured to be in interchangeable association with said vessel or container.

19. The apparatus of claim 18 , further comprising a stopper, wherein said at least one emitter is disposed on said stopper, and said stopper is configured to provide the interchangeable association with said vessel or container.

20. The apparatus of claim 19 , wherein said stopper is configured to provide the interchangeable association with said at least one emitter.

21. The apparatus of claim 18 , further comprising a stopper, wherein said stopper comprises an inner stopper and an outer stopper, wherein said outer stopper is in communication with said vessel or container, and wherein:
said at least one emitter is disposed on said inner stopper, and
said inner stopper is interchangeably disposed with said outer stopper.

22. The apparatus of claim 21 , wherein said disposal of said at least one emitter is provided by an emitter surface or emitter substrate.

23. The apparatus of claim 21 , wherein said at least one emitter that is disposed on said inner stopper is interchangeably disposed with said inner stopper.

24. The apparatus of claim 23 , wherein said disposal of said at least one emitter is provided by an emitter surface or emitter substrate.

25. The apparatus of claim 21 , wherein said outer stopper in communication with said vessel or container is interchangeably disposed with said vessel or container.

26. The apparatus of claim 25 , wherein said at least one emitter that is disposed on said inner stopper is interchangeably disposed with said inner stopper.

27. The apparatus of claim 26 , wherein said disposal of said at least one emitter is provided by an emitter surface or emitter substrate.

28. The apparatus of claim 12 , wherein said at least one receptor is configured to be in interchangeable association with said vessel or container.

29. The apparatus of claim 28 , further comprising a base, wherein said at least one receptor is disposed on said base, and said base is configured to provide the interchangeable association with said vessel or container.

30. The apparatus of claim 29 , wherein said base is configured to provide the interchangeable association with said at least one receptor.

31. The apparatus of claim 28 , further comprising a base, wherein said base comprises an inner base and an outer base, wherein said outer base is in communication with said vessel or container, and wherein:
said at least one receptor is disposed on said inner base, and
said inner base is interchangeably disposed with said outer base.

32. The apparatus of claim 31 , wherein said at least one receptor that is disposed on said inner base is interchangeably disposed with said inner base.

33. The apparatus of claim 31 , wherein said outer base in communication with said vessel or container is interchangeably disposed with said vessel or container.

34. The apparatus of claim 33 , wherein said at least one receptor that is disposed on said inner base is interchangeably disposed with said inner base.

35. The

Assignees

• Univ Virginia Patent Foundation

Inventors

• Papin Jason A
• Dougherty Bonnie V
• Geldert Alisha Mae
• Seitter Kevin P

Classifications

• G01N21/51 Primary

  inside a container, e.g. in an ampoule (G01N21/53 takes precedence) · CPC title

• C12M23/08

  Flask, bottle or test tube · CPC title

• C12M41/46

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title

• G01N21/59

  Transmissivity (G01N21/25 takes precedence) · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title