Devices and methods for processing a biological sample
US-2015177111-A1 · Jun 25, 2015 · US
Methods and devices for producing cellular suspensions from tissue samples
US10900876B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10900876-B2 |
| Application number | US-201716082513-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 7, 2017 |
| Priority date | Mar 10, 2016 |
| Publication date | Jan 26, 2021 |
| Grant date | Jan 26, 2021 |
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- Title
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Abstract
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Aspects of the present disclosure include methods of producing a cellular suspension from a tissue sample by applying resonant acoustic energy to a container comprising the tissue sample in a manner sufficient to produce a cellular suspension from the tissue sample. Resonant acoustic mixers and kits for use in producing a cellular suspension from a tissue sample are also provided.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of producing a cellular suspension from a tissue sample, the method comprising: applying resonant acoustic energy to a container comprising the tissue sample at a frequency ranging from 20 Hz to 20,000 Hz to dissociate cells from the tissue sample and produce live dissociated cells in a cellular suspension from the tissue sample. 2. The method according to claim 1 , wherein the container is a sealed container. 3. The method according to claim 1 , wherein the tissue sample is a mammalian tissue sample. 4. The method according to claim 3 , wherein the tissue sample is a biopsy sample. 5. The method according to claim 4 , wherein the biopsy sample is a tumor biopsy sample. 6. The method according to claim 1 , wherein the method further comprises manipulating the cellular suspension. 7. The method according to claim 6 , wherein the manipulating comprises flow cytometrically processing the cellular suspension. 8. The method according to claim 7 , wherein the flow cytometrically processing the cellular suspension comprises sorting the cellular suspension. 9. The method according to claim 1 , wherein the method further comprises assaying a cell of the cellular suspension. 10. The method according to claim 9 , where the assaying comprises nucleic acid analysis. 11. The method according to claim 1 , wherein the container further comprises at least one of an enzymatic dissolution agent and a milling agent. 12. The method according to claim 1 , wherein the resonant acoustic energy is supplied by cyclic linear displacement of the container.
Assignees
Inventors
Classifications
Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title
by enzymatic treatment · CPC title
Vibrating; Shaking; Tilting · CPC title
- B01F31/80Primary
Mixing by means of high-frequency vibrations above one kHz, e.g. ultrasonic vibrations · CPC title
Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials · CPC title
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- What does patent US10900876B2 cover?
- Aspects of the present disclosure include methods of producing a cellular suspension from a tissue sample by applying resonant acoustic energy to a container comprising the tissue sample in a manner sufficient to produce a cellular suspension from the tissue sample. Resonant acoustic mixers and kits for use in producing a cellular suspension from a tissue sample are also provided.
- Who is the assignee on this patent?
- Becton Dickinson Co
- What technology area does this patent fall under?
- Primary CPC classification B01F31/80. Mapped technology areas include Operations & Transport.
- When was this patent published?
- Publication date Tue Jan 26 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).