Microfluidic nucleic acid analysis
US-9579650-B2 · Feb 28, 2017 · US
US10898871B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10898871-B2 |
| Application number | US-201816025744-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 2, 2018 |
| Priority date | Jul 2, 2018 |
| Publication date | Jan 26, 2021 |
| Grant date | Jan 26, 2021 |
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A MEMS multiplexing system including: first and second fluid inputs; and a mixing network. The mixing network including: a first channel to receive the first fluid input; a second channel to receive the second fluid input; a multiplexing valve communicating with the first channel and the second channel, the multiplexing valve to cause the transport of the first fluid into the second channel so as to form a first interleaved fluid downstream from the multiplexing valve in the second channel and to cause the transport of the second fluid into the first channel so as to form a second interleaved fluid downstream from the multiplexing valve in the first channel; and the first channel and the second channel intersecting downstream from the valve so as to force mixing of the first interleaved fluid and the second interleaved fluid.
Opening claim text (preview).
What is claimed is: 1. A micro electrical mechanical system (MEMS) multiplexing system comprising: a first fluid input in a mixing system; a second fluid input in the mixing system; a mixing network in the mixing system comprising: a first channel to receive the first fluid input; a second channel to receive the second fluid input; a multiplexing valve communicating with the first channel and the second channel, the multiplexing valve to cause the transport of the first fluid into the second channel so as to form a first interleaved fluid downstream from the multiplexing valve in the second channel and to cause the transport of the second fluid into the first channel so as to form a second interleaved fluid downstream from the multiplexing valve in the first channel; the first channel and the second channel intersecting downstream from the valve so as to force mixing of the first interleaved fluid and the second interleaved fluid to form a mixture of the first interleaved fluid and the second interleaved fluid; and an output channel to discharge the mixture of the first interleaved fluid and the second interleaved fluid. 2. The system of claim 1 wherein the first channel and the second channel intersecting at an acute angle. 3. The system of claim 2 wherein the acute angle is 60 to 80 degrees. 4. The system of claim 1 further comprising an optical sensor in the mixing network to monitor the composition of the mixture of the first interleaved fluid and the second interleaved fluid. 5. The system of claim 1 further comprising a reservoir and wherein the output channel to output the mixture transports the mixture to the reservoir. 6. The system of claim 5 wherein the reservoir provides an input to at least one of the nodes. 7. A micro electrical mechanical system (MEMS) multiplexing system comprising: a first fluid input; a second fluid input; a mixing network comprising: a first stage comprising at least first and second mixing nodes, each node comprising: a first channel to receive the first fluid input; a second channel to receive the second fluid input; a multiplexing valve communicating with the first channel and the second channel, the multiplexing valve to cause the transport of the first fluid into the second channel so as to form a first interleaved fluid downstream from the multiplexing valve in the second channel and to cause the transport of the second fluid into the first channel so as to form a second interleaved fluid downstream from the multiplexing valve in the first channel; the first channel and the second channel intersecting downstream from the multiplexing valve so as to force mixing of the first interleaved fluid and the second interleaved fluid so as to form a node mixture of the first interleaved fluid and the second interleaved fluid; an output channel to discharge to a second stage the node mixture; a second stage comprising: a third channel to receive the node mixture from the first node; a fourth channel to receive the node mixture from the second node; a second stage multiplexing valve communicating with the third channel and the fourth channel, the second stage multiplexing valve to cause the transport of the first node mixture from the third channel into the fourth channel so as to form a third interleaved fluid downstream from the second stage multiplexing valve in the fourth channel and to cause the transport of the second node mixture from the fourth channel into the third channel so as to form a fourth interleaved fluid downstream from the second stage multiplexing valve in the first channel; the third channel and the fourth channel intersecting downstream from the second stage multiplexing valve so as to force mixing of the third interleaved fluid and the fourth interleaved fluid to form a second stage mixture of the third interleaved fluid and the fourth interleaved fluid; and an output channel to discharge the second stage mixture. 8. The system of claim 7 wherein the first channel and the second channel intersecting at a first acute angle and the third channel and the fourth channel intersecting at a second acute angle. 9. The system of claim 8 wherein the first acute and the second acute angle is 60 to 80 degrees. 10. The system of claim 7 further comprising optical sensors in the mixing network to monitor the composition of the node mixture and the composition of the second stage mixture. 11. The system of claim 7 wherein the first stage and the second stage form a mixing unit and wherein there are at least a first mixing unit and a second mixing unit and further comprising a third stage, the third stage comprising: a fifth channel to receive the second stage mixture from the first mixing unit; a sixth channel to receive the second stage mixture from the second mixing unit; a third stage multiplexing valve communicating with the fifth channel and the sixth channel, the third stage multiplexing valve to cause the transport of the second stage mixture from the fifth channel into the sixth channel so as to form a fifth interleaved fluid downstream from the third stage multiplexing valve in the sixth channel and to cause the transport of the second stage mixture from the sixth channel into the fifth channel so as to form a sixth interleaved fluid downstream from the third stage multiplexing valve in the fifth channel; the fifth channel and the sixth channel intersecting downstream from the third stage multiplexing valve so as to force mixing of the fifth interleaved fluid and the sixth interleaved fluid to form a third stage mixture of the fifth interleaved fluid and the sixth interleaved fluid; and an output channel to discharge the third stage mixture. 12. The system of claim 7 wherein the fifth channel and the sixth channel intersecting at a third acute angle. 13. The system of claim 12 wherein the third acute angle is 60 to 80 degrees. 14. The system of claim 7 further comprising a reservoir and wherein the output channel to output the second stage mixture transports the second stage mixture to the reservoir. 15. The system of claim 14 wherein the reservoir provides an input to at least one of the nodes. 16. The system of claim 11 further comprising an intermediate stage between the mixing unit and the third stage, the intermediate stage comprising a node to receive in the second channel of the node as an input the discharge from the mixing unit and providing fresh fluid as an input to the first channel of the node. 17. A micro electrical mechanical system (MEMS) multiplexing mixing network comprising: a first channel having a first fluid; a second channel having a second fluid; a multiplexing valve communicating with the first channel and the second channel, the multiplexing valve to cause the transport of the first fluid into the second channel so as to form a first interleaved fluid downstream from the multiplexing valve in the second channel and to cause the transport of the second fluid into the first channel so as to form a second interleaved fluid downstream from the multiplexing valve in the first channel; the first channel and the second channel intersecting downstream from the valve so as to force mixing of the first interleaved fluid and the second interleaved fluid to form a mixture of the first interleaved fluid and the second interleaved fluid; and an output channel to discharge the mixture of the first interleaved fluid and the second interleaved fluid. 18. The mixing network of claim 17 wherein the first channel and the second channel intersectin
using mixing means not otherwise provided for (B01F25/00, B01F27/00, B01F29/00, B01F31/00, B01F33/301 - B01F33/3045, B01F33/40 and B01F33/45 take precedence) · CPC title
using valves, gates, orifices or openings · CPC title
using valves, gates, orifices or openings · CPC title
Concentration, pH, pOH, p(ION) or oxygen-demand (B01F35/2133 takes precedence) · CPC title
Micromixers · CPC title
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