Vibrio parahaemolyticus bacteriophage Vib-PAP-5 and use thereof for suppressing proliferation of Vibrio parahaemolyticus bacteria

Patent metadata

Field	Value
Publication number	US-10898531-B2
Application number	US-201716309663-A
Country	US
Kind code	B2
Filing date	Jun 13, 2017
Priority date	Jun 14, 2016
Publication date	Jan 26, 2021
Grant date	Jan 26, 2021

Abstract

The present invention relates to a Myoviridae bacteriophage Vib-PAP-5 (accession number KCTC 13029BP) isolated from nature characterized by having a capability for specifically killing Vibrio parahaemolyticus bacteria and having a genome expressed by the SEQ ID NO:1, and to a method for preventing and treating infections from Vibrio parahaemolyticus bacteria by means of a composition comprising the Myoviridae bacteriophage Vib-PAP-5 as an active ingredient.

First claim

The invention claimed is:

1. A method for treating a Vibrio parahaemolyticus infection, the method comprising: administering to an animal other than a human a composition comprising an isolated Myoviridae bacteriophage Vib-PAP-5 (Accession number: KCTC 13029BP) that can kill Vibrio parahaemolyticus specifically as an active ingredient, wherein the Myovirdae bacteriophage Vib-PAP-5 is prepared by bacterial culture with inoculum of bacteriophage Vib-PAP-5 and comprises a genome encoded by the nucleotide sequence of SEQ ID NO:1, wherein the composition is administered as a feed additive or a medicine bath agent.

2. The method of claim 1 , wherein said composition further comprises a pharmaceutically acceptable carrier.

3. The method of claim 2 , wherein said composition for the medicine bath agent comprises the bacteriophage Vib-PAP-5 at a concentration of 1×10 1 pfu/ml to 1×10 30 pfu/ml.

4. The method of claim 2 , wherein said composition for the food additive comprises the bacteriophage Vib-PAP-5 at a concentration of 1×10 1 pfu/g to 1×10 30 pfu/g.

Assignees

• Intron Biotechnology Inc

Inventors

• Yoon Seong Jun
• Kang Sang Hyeon
• Jun Soo Youn
• Kwon An Sung

Classifications

• C12N2795/10131

  Uses of virus other than therapeutic or vaccine, e.g. disinfectant · CPC title

• C12N2795/10121

  Viruses as such, e.g. new isolates, mutants or their genomic sequences · CPC title

• A23K20/153

  Nucleic acids; Hydrolysis products or derivatives thereof · CPC title

• C12N2795/10132

  Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent · CPC title

• A23K50/80

  for aquatic animals, e.g. fish, crustaceans or molluscs · CPC title