Aldehyde synthase gene, recombinant microorganism comprising the same, and method for producing alkane using the same

Patent metadata

Field	Value
Publication number	US-10894967-B2
Application number	US-202016807495-A
Country	US
Kind code	B2
Filing date	Mar 3, 2020
Priority date	Mar 4, 2019
Publication date	Jan 19, 2021
Grant date	Jan 19, 2021

Abstract

The present disclosure provides a novel aldehyde synthase gene having activity of synthesizing a medium-chain aldehyde. Such gene encodes a protein comprising the amino acid sequence as shown in SEQ ID NO: 2.

First claim

What is claimed is:

1. An expression vector encoding an aldehyde synthase comprising an aldehyde synthase gene and a regulatory region that regulates expression of such gene, wherein the aldehyde synthase gene comprises a nucleic acid sequence that has 85% or higher sequence identity to the nucleic acid sequence as shown in SEQ ID NO: 1 and further conserves encoding valine 36 and valine 322 and wherein the aldehyde synthase has activity for synthesizing an aldehyde from an alcohol.

2. A purified aldehyde synthase encoded by the aldehyde synthase gene of the expression vector according to claim 1 .

3. An aldehyde synthase expressed by the expression vector according to claim 1 , wherein the aldehyde synthase comprises an amino acid sequence as shown in SEQ ID NO: 2.

4. A recombinant microorganism prepared by introducing the expression vector according to claim 1 into a host microorganism.

5. The recombinant microorganism according to claim 4 , wherein the host microorganism is a bacterium of Escherichia coli or Klebsiella.

6. The recombinant microorganism according to claim 4 , which further comprises a decarbonylase gene, a ferredoxin gene, and a ferredoxin reductase gene introduced thereinto.

7. A method for producing an alkane comprising culturing the recombinant microorganism according to claim 4 .

8. The method for producing an alkane according to claim 7 , wherein the recombinant microorganism is cultured in an alkaline medium.

9. The method for producing an alkane according to claim 7 , which further comprises recovering an alkane from a medium in which the recombinant microorganism is cultured.

10. The method for producing an alkane according to claim 7 , which further comprises recovering an alkane from a medium in which the recombinant microorganism is cultured and purifying the recovered alkane.

11. The method for producing an alkane according to claim 7 , which further comprises producing an alkane having 9 to 20 carbon atoms.

12. A method for producing an alkane comprising culturing the recombinant microorganism according to claim 5 .

13. The method for producing an alkane according to claim 12 , wherein the recombinant microorganism is cultured in an alkaline medium.

14. The method for producing an alkane according to claim 12 , which further comprises recovering an alkane from a medium in which the recombinant microorganism is cultured.

15. The method for producing an alkane according to claim 12 , which further comprises recovering an alkane from a medium in which the recombinant microorganism is cultured and purifying the recovered alkane.

16. The method for producing an alkane according to claim 12 , which further comprises producing an alkane having 9 to 20 carbon atoms.

17. A method for producing an alkane comprising culturing the recombinant microorganism according to claim 6 .

18. The method for producing an alkane according to claim 17 , wherein the recombinant microorganism is cultured in an alkaline medium.

19. The method for producing an alkane according to claim 17 , which further comprises recovering an alkane from a medium in which the recombinant microorganism is cultured.

20. The method for producing an alkane according to claim 17 , which further comprises recovering an alkane from a medium in which the recombinant microorganism is cultured and purifying the recovered alkane.

Assignees

• Muramatsu Masayoshi
• Obata Shusei
• Ito Masakazu
• Ogawa Jun
• Kishino Shigenobu
• Toyota Motor Co Ltd
• Univ Kyoto

Inventors

• Muramatsu Masayoshi
• Obata Shusei
• Ito Masakazu
• Ogawa Jun
• Kishino Shigenobu

Classifications

• C12R2001/22

  Klebsiella · CPC title

• C12R2001/19

  Escherichia coli · CPC title

• C12N1/205

  Bacterial isolates · CPC title

• C12P7/40

  containing a carboxyl group {including Peroxycarboxylic acids} · CPC title

• C12P7/24

  containing a carbonyl group · CPC title