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US-9206403-B1 · Dec 8, 2015 · US
Enzyme variants with improved ester synthase properties
US10894953B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10894953-B2 |
| Application number | US-201815873977-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 18, 2018 |
| Priority date | Sep 14, 2012 |
| Publication date | Jan 19, 2021 |
| Grant date | Jan 19, 2021 |
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- Title
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Abstract
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The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells.
First claim
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We claim: 1. A variant ester synthase polypeptide comprising a variant of SEQ ID NO: 67, wherein said variant ester synthase polypeptide the variant of SEQ ID NO: 67 is genetically engineered to have at least one substitution mutation at an amino acid position selected from the group of G4R, T5P, T5S, D7N, S15G, T24M, T24W, L30H, G33S, L39A, L39M, L39S, R40S, D41A, D41G, D41H, D41Y, V43K, V43S, T44F, A73Q, V76L, D77A, K78F, K78W, I80V, R98D, E99Q, G101L, I102R, P111D, P111G, P111S, H122S, R131M, I146K, I146L, I146R, S147A, V149L, R150P, V155G, N164R, P166S, T170R, V171E, V171F, V171H, V171R, V171W, R172S, R172W, P173W, R177V, A179V, D182G, E184F, E184G, E184L, E184R, E184S, A185L, A185M, S186T, V187G, V187R, P188R, A190P, A190R, A190W, S192A, S192L, S192V, Q193R, Q193S, M195G, A197T, A197V, Q201A, Q201V, Q201W, A202L, D203R, P206F, R207A, G212A, G212L, V219L, V234C, H239G, T242K, T242R, A243R, Q244G, R246A, R246G, R246L, R246Q, R246V, R246W, K258R, S264D, S264V, S264W, S267G, A285R, P294G, V301A, N302G, I303G, I303R, I303W, R304W, A306G, D307F, D307G, D307L, D307N, D307R, D307V, E309A, E309G, E309S, G310H, G310R, G310V, T311S, T313S, Q314G, I315F, S316G, F317W, I319G, A320C, A323G, D328F, Q334S, Q348A, Q348R, K349A, K349C, K349H, K349Q, P351G, K352I, K352N, S353T, T356G, T356W, Q357V, M360Q, M360R, M360S, M360W, Y366G, Y366W, G375A, G375V, G375S, V381F, E393R, E393W, T395E, V409L, L411A, I420V, S424G, S424Q, S442E, S442G, M443G, A447C, A447I, A447L, L454V, D455E, L457Y, E458W, I461G, K466N, A468G, K472T and K472*, wherein said variant ester synthase polypeptide has improved fatty acid ester activity compared to a corresponding wild type polypeptide comprising the amino acid sequence of SEQ ID NO: 67.
Assignees
Inventors
Classifications
using bio-feedstock · CPC title
Thioester hydrolases (3.1.2) · CPC title
Fatty-acyl-ethyl-ester synthase (3.1.1.67) · CPC title
- C12N9/18Primary
Carboxylic ester hydrolases {(3.1.1)} · CPC title
acting on ester bonds (3.1) · CPC title
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- What does patent US10894953B2 cover?
- The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells.
- Who is the assignee on this patent?
- Genomatica Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/18. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 19 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).