Antibodies against ox40 and uses thereof
US-2016347849-A1 · Dec 1, 2016 · US
Antibody Fc mutants with ablated effector functions
US10894836B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10894836-B2 |
| Application number | US-201816037304-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 17, 2018 |
| Priority date | Nov 30, 2009 |
| Publication date | Jan 19, 2021 |
| Grant date | Jan 19, 2021 |
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- Title
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Abstract
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Antibody and other Fc-containing molecules with variations in the Fc region with reduced binding to Fc gamma receptors and resulting activity and can be used in the treatment of various diseases and disorders.
First claim
Opening claim text (preview).
What is claimed: 1. An isolated polynucleotide encoding an Fc-containing molecule, comprising a mutated IgG1 Fc domain and having binding reduced by at least 800 fold or undetectable binding to a Fcγ receptor (FcγR), selected from the group consisting of FcγRI, FcγRIIa-H131 variant, FcγRIIa-R131 variant, FcγRIIb, and FcγRIII-V158 variant, as compared to an Fc-containing molecule with a wild type IgG1 Fc domain, wherein the mutated IgG1 Fc comprises the mutations L234A, L235A, G237A P238S, H268A, A330S, and P331S, wherein the binding is measured by conjugating the Fc-containing molecule and the FcγR to a donor and an acceptor beads and by measuring the energy transfer between the donor and the acceptor beads using ALPHASCREEN® assay. 2. The polynucleotide of claim 1 , wherein the polynucleotide encodes a molecule comprising is an antibody, an antibody fragment or an Fc region. 3. A vector comprising the polynucleotide of claim 1 or 2 . 4. An isolated host cell comprising the vector of claim 3 . 5. A method of making an Fc-containing molecule, comprising culturing the host cell of claim 4 under conditions wherein the Fc mutant-containing molecule is expressed, purifying the expressed Fc mutant-containing molecule, and measuring the reduced binding (by at least 800 fold) or undetectable binding of the Fc mutant-containing molecule to the Fcγ receptor (FcγR), selected from the group consisting of FcγRI, FcγRIIa-H131 variant, FcγRIIa-R131 variant, FcγRIIb, and FcγRIII-V158 variant, as compared to an Fc-containing molecule with a wild type IgG1 Fc domain.
Assignees
Inventors
Classifications
Decreased effector function due to an Fc-modification · CPC title
Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation · CPC title
Antibody-dependent cellular cytotoxicity [ADCC] · CPC title
Agonist effect on antigen · CPC title
CH2 domain · CPC title
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- What does patent US10894836B2 cover?
- Antibody and other Fc-containing molecules with variations in the Fc region with reduced binding to Fc gamma receptors and resulting activity and can be used in the treatment of various diseases and disorders.
- Who is the assignee on this patent?
- Janssen Biotech Inc
- What technology area does this patent fall under?
- Primary CPC classification C07K16/32. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 19 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 12 related publications on this page (citations in our corpus or others sharing the same primary CPC).