Method of isolating biochemical molecules on microarray substrate

Patent metadata

Field	Value
Publication number	US-10883136-B2
Application number	US-201314650311-A
Country	US
Kind code	B2
Filing date	Dec 9, 2013
Priority date	Dec 7, 2012
Publication date	Jan 5, 2021
Grant date	Jan 5, 2021

Abstract

Provided is a method of isolating biochemical molecules on a microarray substrate, the method including providing a microarray substrate to which clusters of different kinds of biochemical molecules being classified by individual spot units are attached, the individual spots being regularly arranged thereon; obtaining location information of the individual spot in which a desired cluster among clusters of the biochemical molecules locates; locating an extraction tool for applying energy to isolate the desired cluster according to the location information; and isolating the desired cluster from the microarray substrate by applying energy in a contact or non-contact manner using the extraction tool.

First claim

The invention claimed is:

1. A method of isolating biochemical molecules on a microarray substrate, the method comprising:
providing a microarray substrate to which clusters of different kinds of biochemical molecules being classified by individual spot units are attached, the individual spots being regularly arranged thereon,
wherein the microarray substrate is one of a substrate including a sacrificial layer, a substrate surface-coated with a sacrificial layer, and combinations thereof;
obtaining location information of the individual spot in which a desired cluster among clusters of the biochemical molecules locates;
locating an extraction tool for applying energy to selectively isolate the desired cluster according to the location information; and
selectively isolating the desired cluster from the sacrificial layer of the microarray substrate by applying energy in a contact or non-contact manner using the extraction tool, such that the desired cluster is selectively and directly transferred to a selected well in a reservoir well plate below each of the individual spot units when the desired cluster is severed from the microarray substrate,
wherein the microarray substrate is irradiated with a pulse laser to apply energy.

2. The method according to claim 1 , wherein pulse laser ablation or radiation pressure ejection is performed by irradiation with the pulse laser.

3. The method according to claim 1 , wherein the pulse laser has a wavelength of 10 to 10,000 nm.

4. The method according to claim 1 , wherein the pulse laser has a pulse duration of 1 as to 1 ms.

5. The method according to claim 1 , wherein the biochemical molecules comprise nucleic acids, polypeptides, peptides and combinations thereof.

6. The method according to claim 5 , wherein the nucleic acids comprise DNA, RNA, PNA, LNA, GNA, TNA, XNA, synthetic nucleic acids, modified nucleic acids and combinations thereof.

7. The method according to claim 5 , wherein a 3′ terminal of the nucleic acids is fixed on the microarray substrate via a linker.

8. The method according to claim 1 , wherein the microarray substrate is manufactured through solid-phase synthesis to sequentially synthesize monomers of the biochemical molecules on a surface of the microarray substrate.

9. The method according to claim 1 , wherein other molecules or other cells are introduced to the microarray via the biochemical molecules synthesized on the microarray.

10. The method according to claim 1 , wherein the isolating comprises transferring the desired cluster to a reservoir individually.

11. The method according to claim 10 , wherein the isolating is performed by partially or entirely transferring the cluster to at least one reservoir individually.

12. The method according to claim 1 , wherein the pulse laser is irradiated to sever a connection between the biochemical molecules and the microarray substrate.

13. The method according to claim 1 , further comprising amplifying the desired cluster isolated from the microarray substrate.

14. The method according to claim 1 , further comprising using the desired cluster isolated from the microarray substrate or a chemical reaction product thereof in a sequencing process or a gene synthesis process.

15. The method according to claim 1 , wherein, in the step of selectively isolating the desired cluster, the desired cluster is isolated by radiation pressure ejection.

16. The method according to claim 1 , wherein the microarray substrate comprises a front surface in direct contact with the clusters and a back surface opposite to the front surface, and wherein the step of selectively isolating the desired cluster applies the energy to the microarray substrate from the back surface.

Assignees

• Snu R&Db Foundation

Inventors

• Kwon Sunghoon
• Ryu Taehoon
• Choi Yeongjae
• Jung Yushin
• Kim Hyoki
• Lee Howon

Classifications

• B01J19/0046 Primary

  Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00) · CPC title

• B01J2219/00452

  Means for the recovery of reactants or products · CPC title

• B01J2219/00716

  Heat activated synthesis · CPC title

• B01J2219/00608

  DNA chips · CPC title

• B01J2219/0054

  Means for coding or tagging the apparatus or the reagents · CPC title