Feruloyl-CoA:monolignol transferases

Patent metadata

Field	Value
Publication number	US-10883089-B2
Application number	US-201815945463-A
Country	US
Kind code	B2
Filing date	Apr 4, 2018
Priority date	Apr 4, 2017
Publication date	Jan 5, 2021
Grant date	Jan 5, 2021

Abstract

The invention relates to feruloyl-CoA:monolignol transferase enzymes and nucleic acids encoding the feruloyl-CoA:monolignol transferase enzymes. The enzymes and/or the nucleic acids enable incorporation of monolignol ferulates into the lignin of plants. The monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate.

First claim

We claim:

1. A transgenic plant comprising an isolated nucleic acid molecule, wherein the isolated nucleic acid molecule comprises a nucleotide sequence encoding a polypeptide having feruloyl-CoA:monolignol transferase activity, wherein the polypeptide comprises an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:2, wherein the nucleotide sequence is operably linked to a heterologous promoter functional or active in a plant cell, and wherein expression of the polypeptide in the transgenic plant increases percent of monolignol ferulates in the transgenic plant's lignin as compared to a control plant of the same species lacking the isolated nucleic acid molecule and grown under identical conditions.

2. The transgenic plant of claim 1 , wherein the transgenic plant does not have an increased percent of monolignol coumarates in the transgenic plant's lignin as compared to the control plant.

3. The transgenic plant of claim 1 , wherein genome of the transgenic plant is stably transformed with the isolated nucleic acid molecule.

4. The transgenic plant of claim 1 , wherein the heterologous promoter is functional or active during plant growth or development.

5. The transgenic plant of claim 1 , wherein the heterologous promoter is functional or active in a woody tissue of a plant.

6. A transgenic seed obtained from the transgenic plant of claim 1 , wherein the transgenic seed comprises the isolated nucleic acid molecule.

7. The transgenic plant of claim 1 , wherein the polypeptide has the amino acid sequence as set forth in SEQ ID NO:2.

8. A method for increasing a content of monolignol ferulates in lignin within a plant, comprising:
(a) planting the transgenic seed of claim 6 ; and
(b) cultivating a transgenic plant germinated from the transgenic seed, wherein expression of the polypeptide in the germinated transgenic plant increases the content of monolignol ferulates in the lignin within the germinated transgenic plant as compared to a control plant lacking the isolated nucleic acid molecule and grown under identical growth conditions.

9. A method of obtaining a plant having increased content of monolignol ferulates in lignin within the plant, comprising the steps:
(i) stably transforming plant cells with an isolated nucleic acid molecule, wherein the isolated nucleic acid molecule comprises a nucleotide sequence encoding a polypeptide having feruloyl-CoA:monolignol transferase activity, wherein the polypeptide comprises an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:2, wherein the nucleotide sequence is operably linked to a heterologous promoter functional or active in a plant cell; and
(ii) regenerating a transformed plant with the stably transformed plant cells from step (i), wherein genome of the regenerated transgenic plant is stably transformed with the isolated nucleic acid molecule, and wherein the regenerated transgenic plant has increased percent of monolignol ferulates in the regenerated transgenic plant's lignin as compared to a control plant of the same species lacking the isolated nucleic acid molecule and grown under identical conditions.

10. The method of claim 9 , wherein the polypeptide has the amino acid sequence as set forth in SEQ ID NO:2.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Ralph John
• Karlen Steven D
• Smith Rebecca Anne
• Fox Brian
• Beebe Emily
• Bingman Craig

Classifications

• C12N15/8255

  involving lignin biosynthesis · CPC title

• C12N9/1029 Primary

  transferring groups other than amino-acyl groups (2.3.1) · CPC title