System with reusable column with resin packing and unpacking procedures and sample analysis

Patent metadata

Field	Value
Publication number	US-10881985-B1
Application number	US-201715836305-A
Country	US
Kind code	B1
Filing date	Dec 8, 2017
Priority date	Dec 8, 2016
Publication date	Jan 5, 2021
Grant date	Jan 5, 2021

Abstract

Systems and methods for automatically packing resin into and unpacking resin from a reusable separation column for sample analysis are described. A method embodiment includes, but is not limited to, introducing a slurry of resin to a reusable sample separation column to pack the reusable column with resin; introducing a sample solution to the reusable sample separation column; and unpacking the resin from the reusable sample separation column with a flow of liquid unpacking reagent and gaseous material.

First claim

What is claimed is:

1. A method comprising:
introducing, via pump operation, a first slurry of resin to a first end of a first reusable sample separation column coupled with a valve system to pack resin from the first slurry of resin into the first reusable sample separation column when the valve system is in a first resin packing configuration;
introducing, via pump operation, a second slurry of resin to a first end of a second reusable sample separation column coupled with the valve system to pack resin from the second slurry of resin into the second reusable sample separation column when the valve system is in a second resin packing configuration;
introducing, via pump operation, a sample solution to the first reusable sample separation column and the second reusable sample separation column when the valve system is in a sample load configuration;
mixing a gas and an unpacking reagent in the valve system;
introducing, via pump operation, the mixed gas and unpacking reagent to a second end of the first reusable sample separation column to unpack resin from the first reusable sample separation column and transfer the resin out of the first end of the first reusable sample separation column, wherein the second end of the first reusable sample separation column is distal the first end of the first reusable sample separation column; and
introducing, via pump operation, the mixed gas and unpacking reagent to a second end of the second reusable sample separation column to unpack resin from the second reusable sample separation column and transfer the resin out of the first end of the second reusable sample separation column, wherein the second end of the second reusable sample separation column is distal the first end of the second reusable sample separation column.

2. The method of claim 1 , further comprising:
sensing a level of resin within the first reusable sample separation column with an optical sensor during introduction of the first slurry of resin to the first reusable sample separation column.

3. The method of claim 2 , further comprising:
ceasing introduction of the first slurry of resin to the first reusable sample separation column responsive to an output from the optical sensor.

4. The method of claim 2 , further comprising:
transitioning the valve system from the first resin packing configuration to the second resin packing configuration responsive to an output from the optical sensor.

5. The method of claim 2 , further comprising:
transitioning the valve system from at least one of the first resin packing configuration or the second resin packing configuration to the sample load configuration responsive to an output from the optical sensor.

6. The method of claim 1 , wherein mixing the gas and the unpacking reagent in the valve system includes introducing the gas and the unpacking reagent to a mixing port of a valve of the valve system.

7. The method of claim 1 , wherein introducing, via pump operation, the mixed gas and unpacking reagent to the second end of the first reusable sample separation column includes inducing a turbulent environment within the first reusable sample separation column.

8. The method of claim 1 , wherein the gas is an inert gas.

9. The method of claim 1 , wherein the gas is argon.

10. The method of claim 1 , wherein the first slurry of resin includes a same resin type as the second slurry of resin.

11. The method of claim 1 , wherein the first slurry of resin differs from the second slurry of resin on the basis of at least one of resin type or resin concentration.

12. A method comprising:
introducing, via pump operation, a slurry of resin to a first end of a reusable sample separation column coupled with a valve system to pack resin from the slurry of resin into the reusable sample separation column when the valve system is in a resin packing configuration;
introducing, via pump operation, a sample solution to the reusable sample separation column when the valve system is in a sample load configuration;
mixing a gas and an unpacking reagent in the valve system; and
introducing, via pump operation, the mixed gas and unpacking reagent to a second end of the reusable sample separation column to unpack resin from the reusable sample separation column and transfer the resin out of the first end of the reusable sample separation column, wherein the second end of the reusable sample separation column is distal the first end of the reusable sample separation column.

13. The method of claim 12 , further comprising:
sensing a level of resin within the reusable sample separation column with an optical sensor during introduction of the slurry of resin to the reusable sample separation column.

14. The method of claim 13 , further comprising:
ceasing introduction of the slurry of resin to the reusable sample separation column responsive to an output from the optical sensor.

15. The method of claim 14 , further comprising:
transitioning the valve system from the resin packing configuration to the sample load configuration responsive to an output from the optical sensor.

16. The method of claim 12 , wherein mixing the gas and the unpacking reagent in the valve system includes introducing the gas and the unpacking reagent to a mixing port of a valve of the valve system.

17. The method of claim 12 , wherein introducing, via pump operation, the mixed gas and unpacking reagent to the second end of the reusable sample separation column includes inducing a turbulent environment within the reusable sample separation column.

18. The method of claim 12 , wherein the gas is an inert gas.

19. The method of claim 12 , wherein the gas is argon.

Assignees

• Elemental Scientific Inc

Inventors

• Field Michael Paul

Classifications

• G01N2030/565

  slurry packing · CPC title

• G01N30/8658

  Optimising operation parameters · CPC title

• G01N30/56

  Packing methods or coating methods · CPC title

• B01D15/206 Primary

  Packing or coating · CPC title

• B01D15/1885

  placed in parallel · CPC title