Fiber sampler for recovery of bioaerosols and particles

Patent metadata

Field	Value
Publication number	US-10876145-B2
Application number	US-202016884764-A
Country	US
Kind code	B2
Filing date	May 27, 2020
Priority date	Feb 17, 2012
Publication date	Dec 29, 2020
Grant date	Dec 29, 2020

Abstract

An aerosol collection system and method. The system includes a bio-aerosol delivery device configured to supply bioparticles in a gas stream, a moisture exchange device including a partition member coupled to the gas stream and configured to humidify or dehumidify the bioparticles in the gas stream, and an aerosol collection medium downstream from the moisture exchange device and configured to collect the bioparticles. The method includes delivering bioparticles in a gas stream, humidifying or dehumidifying the bioparticles in the gas stream by transport of water across a partition member and into a vapor phase of the gas stream, and collecting the bioparticles by a collection medium.

First claim

The invention claimed is:

1. An aerosol collection system comprising:
a moisture exchange device comprising at least one partition member separating a first gas stream and a second gas stream, wherein bioparticles in the second gas stream exist in a controlled relative humidity environment by transport of water vapor a) from an exterior side of the at least one partition member to an interior side of the at least one partition member and b) into a vapor phase of the second gas stream including the bioparticles;
a bio-aerosol delivery device configured to supply bioparticles into the second gas stream;
a pressure control device configured to control a pressure in the first gas stream and thereby control the transport of water vapor through the at least one partition member; and
an aerosol collection medium downstream from the moisture exchange device and configured to collect the bioparticles.

2. The system of claim 1 , wherein the at least one partition member comprises at least one of a permeable material, a semi-permeable membrane material, or a polymeric ionomer.

3. The system of claim 1 , wherein the at least one partition member comprises at least one of:
a copolymer of perfluoro-3,6-dioxa-4-methyl-7octene-sulfonic acid and tetrafluoroethylene;
polyethylene oxide, polyvinyl alcohol, cellulose ether and starch) and copolymers thereof; and
polyacrylic acid, polyacrylamide, polyisopropyl acrylamide, polystyrene sulfonic acid, polyvinyl pyridine and polyamino acid and copolymers and salts thereof.

4. The system of claim 1 , wherein the at least one partition member comprises at least one tube of at least one of a permeable material, a semi-permeable membrane material, or a polymeric ionomer.

5. The system of claim 1 , wherein the at least one partition member comprises at least one tube of at least one of:
a copolymer of perfluoro-3,6-dioxa-4-methyl-7octene-sulfonic acid and tetrafluoroethylene;
polyethylene oxide, polyvinyl alcohol, cellulose ether and starch) and copolymers thereof; and
polyacrylic acid, polyacrylamide, polyisopropyl acrylamide, polystyrene sulfonic acid, polyvinyl pyridine and polyamino acid and copolymers and salts thereof.

6. The system of claim 1 , further comprising:
a by-pass gas flow device configured to supply air flow without the entrained bioparticles into the second gas stream in order stabilize at least one of a temperature and relative humidity in the moisture exchange device prior to collecting the bioparticles.

7. The system of claim 1 , further comprising:
a controller configured to control a temperature and a relative humidity along a gas-flow axis of the moisture exchange device.

8. The system of claim 7 , wherein the controller is programmed to adjust the temperature and the relative humidity such that the particles in the second gas stream transition from outside ambient conditions to a target temperature and relative humidity condition.

9. The system of claim 8 , wherein the target temperature and relative humidity condition comprises at least one of:
2° C. to 10° C. and RH of 5 to 95%
2° C. to 10° C. and RH of 70 to 85%;
1° C. to 8° C. and RH of 5 to 95%;
1° C. to 8° C. and RH of 70 to 85%;
2° C. to 6° C. and RH of 5 to 95%;
2° C. to 6° C. and RH of 70 to 85%;
4° C. and RH of 5 to 95%; or
4° C. and RH of 70 to 85%,
where RH is the relative humidity in the second gas stream.

10. The system of claim 8 , wherein the controller is programmed to adjust the temperature and the relative humidity in the second gas stream while transitioning from outside ambient conditions to the target temperature and relative humidity condition along a controlled path of temperature and humidity,
said controlled path comprises a two-stage system including a first stage having the relative humidity in a range from 40-60% at ambient temperature and a second stage below ambient temperature while maintaining the relative humidity in said range from 40-60%.

11. The system of claim 1 , wherein the aerosol collection medium comprises at least one of a flow-through or an impaction device including a plurality of fibers.

12. The system of claim 11 , wherein the aerosol collection medium comprises a viability enhancing material provider disposed in a vicinity of the plurality of fibers and configured to provide a viability enhancing material to the collected bioparticles to maintain viability of the collected bioparticles.

13. The system of claim 12 , wherein the viability enhancing material provider comprises an osmotic material disposed in contact with the plurality of fibers and configured to maintain a relative humidity suitable for said viability of bioparticles.

14. The system of claim 13 , wherein the osmotic material comprises at least one of a water-regulating material configured to provide water or a nutrient supply to the fibers to support biological viability of the collected bioparticles.

15. The system of claim 14 , wherein the nutrient supply comprises a supply of at least one of water, proteins, carbohydrates, sugars, salts, phosphate buffered saline, and tryptic soy broth.

16. The system of claim 1 , wherein the aerosol collection medium comprises a plurality of fibers formed into a fiber mat for collection of the bioaerosol particles.

17. The system of claim 16 , wherein the fibers have an average fiber diameter of less than 10 microns.

18. The system of claim 16 , wherein the fibers have an average fiber diameter of less than 1 micron.

19. The system of claim 16 , wherein the fibers have an average fiber diameter of less than 0.5 micron.

20. The system of claim 1 , wherein the at least one partition member comprises multiple tubes having respective walls separating the first gas stream and the second gas stream.

Assignees

• Res Triangle Inst

Inventors

• Ensor David S
• Walls Howard Jerome
• Foarde Karin K
• Hering Susanne Vera
• Spielman Steven Russel

Classifications

• G01N2001/2217

  using a liquid · CPC title

• G01N1/2202 Primary

  involving separation of sample components during sampling · CPC title

• C12Q1/24 Primary

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title