Helicobacter pylori α-1,3 fucosyltransferase gene and protein with improved soluble protein expression and activity, and thereof application for synthesis of α-1,3 fucosyloligosaccharide
US-10336990-B2 · Jul 2, 2019 · US
Method of producing 2′-fucosyllactose using recombinant Corynebacterium glutamicum
US10876122B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10876122-B2 |
| Application number | US-201916589724-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 1, 2019 |
| Priority date | Apr 25, 2016 |
| Publication date | Dec 29, 2020 |
| Grant date | Dec 29, 2020 |
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- Title
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Abstract
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Provided is a method of producing 2′-fucosyllactose including culturing in a medium supplemented with lactose a recombinant Corynebacterium glutamicum transformed to express α-1,2-fucosyltransferase, transformed to express GDP-D-mannose-4,6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of producing 2′-fucosyllactose comprising culturing in a medium supplemented with lactose a recombinant Corynebacterium glutamicum transformed to express α-1,2-fucosyltransferase, transformed to express GDP-Dmannose-4,6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, and isolating 2′-fucosyllactose from the medium, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase. 2. The method according to claim 1 , wherein the medium further comprises glucose. 3. The method according to claim 2 , wherein the production of the fucosyllactose is carried out by fed-batch culture comprising further supplying glucose or lactose. 4. The method according to claim 1 , wherein the recombinant Corynebacterium glutamicum is transformed to overexpress phosphomannomutase, and transformed to overexpress GTP-mannose-1-phosphate guanylyltransferase.
Assignees
Inventors
Classifications
GDP-mannose 4,6-dehydratase (4.2.1.47), i.e. GMD · CPC title
- C12N15/77Primary
for Corynebacterium; for Brevibacterium · CPC title
- C12N15/52Primary
Genes encoding for enzymes or proenzymes · CPC title
3-Galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase (2.4.1.65), i.e. alpha-1-3 fucosyltransferase · CPC title
Mannose-1-phosphate guanylyltransferase (2.7.7.13) · CPC title
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- What does patent US10876122B2 cover?
- Provided is a method of producing 2′-fucosyllactose including culturing in a medium supplemented with lactose a recombinant Corynebacterium glutamicum transformed to express α-1,2-fucosyltransferase, transformed to express GDP-D-mannose-4,6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum h…
- Who is the assignee on this patent?
- Seoul Nat Univ R&Db Foundation, Advanced Protein Tech Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N15/77. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 29 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).