Engineered enzymes

Patent metadata

Field	Value
Publication number	US-10876102-B2
Application number	US-202016844079-A
Country	US
Kind code	B2
Filing date	Apr 9, 2020
Priority date	Oct 22, 2018
Publication date	Dec 29, 2020
Grant date	Dec 29, 2020

Abstract

The present disclosure provides engineered RNA-guided enzymes for editing live cells.

First claim

We claim:

1. A cocktail of engineered nucleic acid-guided nucleases, wherein one or more nucleases in the cocktail has an altered fidelity relative to the nucleic acid-guided nuclease having the sequence of SEQ ID No. 1 and has a sequence comprising any of SEQ ID Nos. 4-7, and wherein one or more nucleases has editing activity in yeast different than the nucleic acid-guided nuclease having the sequence of SEQ ID No. 1 and has a sequence comprising any of SEQ ID Nos. 69-78 or 79-86.

2. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 69.

3. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 70.

4. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 71.

5. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 72.

6. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 73.

7. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 74.

8. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 75.

9. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 76.

10. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 77.

11. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 78.

12. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 79.

13. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 80.

14. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 81.

15. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 82.

16. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 83.

17. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 84.

18. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 85.

19. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 86.

20. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 4.

21. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 5.

22. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 6.

23. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 7.

Assignees

• Inscripta Inc

Inventors

• Mijts Benjamin
• Kim Juhan
• Mir Aamir
• Seamon Kyle
• Garst Andrew

Classifications

• C12N15/90

  Stable introduction of foreign DNA into chromosome · CPC title

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title

• C12N15/10

  Processes for the isolation, preparation or purification of DNA or RNA (chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34) · CPC title

• C12N9/22 Primary

  Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} · CPC title