Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples

Patent metadata

Field	Value
Publication number	US-10875022-B2
Application number	US-201816124672-A
Country	US
Kind code	B2
Filing date	Sep 7, 2018
Priority date	Jul 13, 2007
Publication date	Dec 29, 2020
Grant date	Dec 29, 2020

Abstract

The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.

First claim

What is claimed is:

1. An apparatus comprising:
a bay configured to removably receive a housing comprising a plurality of process chambers, the plurality of process chambers aligned along a first axis when the housing is received in the bay;
a process chamber of the plurality of process chambers configured to receive liquid containing one or more biomolecules and magnetic particles, the magnetic particles capable of binding to the one or more biomolecules;
a magnetic separator configured to apply a magnetic force to the process chamber when the housing is received in the bay, the magnetic separator comprising one or more magnets along a second axis parallel to the first axis when the housing is received in the bay, wherein the magnetic force applied by the magnetic separator is capable of moving the magnetic particles within the process chamber relative to an inner surface of the process chamber when the liquid containing one or more biomolecules and magnetic particles is received in the process chamber;
a heater assembly configured to apply heat to the process chamber when the housing is received in the bay, the heater assembly comprising one or more heaters along a third axis parallel to the first axis when the housing is received in the bay, wherein the heat applied by the heater assembly is capable of heating the liquid containing one or more biomolecules and magnetic particles in the process chamber when the liquid containing one or more biomolecules and magnetic particles is received in the process chamber;
wherein the magnetic separator and the heater assembly are configured to operate without transporting the liquid containing one or more biomolecules and magnetic particles to a different location to perform either heating by the heater assembly or separation by the magnetic separator when the liquid containing one or more biomolecules and magnetic particles is received in the process chamber; and
a liquid dispenser configured to carry out fluid transfer operations relative to the housing, wherein the fluid transfer operations comprise withdrawing liquid comprising nucleic acids after heating by the heater assembly and separation by the magnetic separator;
wherein the system is configured to add a wash buffer to the process chamber, wherein the system is configured to add a release buffer to the process chamber; and
an optical detection system configured to carry out detection operations, wherein the detection operations comprise detecting nucleic acids in the liquid comprising nucleic acids.

2. The apparatus of claim 1 , wherein the magnetic separator is configured to move from a first position to a second position relative to the process chamber.

3. The apparatus of claim 1 , wherein the magnetic particles comprise microparticles, beads, or microspheres.

4. The apparatus of claim 1 , wherein the biomolecules comprise polynucleotides.

5. The apparatus of claim 1 , further comprising control circuitry configured to control the magnetic separator and the heater assembly.

6. The apparatus of claim 1 , wherein the magnetic separator is configured to be positioned adjacent to a first side of the process chamber when the housing is received in the bay.

7. The apparatus of claim 6 , wherein the heater assembly is configured to be positioned adjacent to a second side of the process chamber when the housing is received in the bay, wherein the first side is opposite the second side.

8. The apparatus of claim 1 , wherein the heater assembly comprises a temperature sensor.

9. The apparatus of claim 1 , wherein the magnetic separator comprises a magnet coupled to a motorized mechanism configured to move the magnet along a fixed axis.

10. The apparatus of claim 1 , wherein the magnetic separator is configured to move a magnet without being in contact with the process chamber.

11. The apparatus of claim 1 , wherein the magnetic separator is configured to separate the magnetic particles by collecting and concentrating, or gathering, the magnetic particles into a location in the process chamber when the liquid containing one or more biomolecules and magnetic particles is received in the process chamber.

12. The apparatus of claim 1 , wherein the magnetic separator comprises a rectangular magnet.

13. The apparatus of claim 1 , wherein the magnetic separator comprises a plurality of magnets.

14. The apparatus of claim 1 , wherein the magnetic separator comprises a magnet wherein one pole of the magnet faces the heater assembly and the other pole of the magnet faces away from the heater assembly.

15. The apparatus of claim 1 , wherein the housing comprises a waste chamber.

16. The apparatus of claim 1 , wherein the magnetic separator is configured to operate in conjunction with the heater assembly to permit successive heating and separation operations to be performed on the magnetic particles in the process chamber when the liquid containing one or more biomolecules and magnetic particles is received in the process chamber.

17. The apparatus of claim 1 , wherein the magnetic separator is configured to move repetitively relative to the process chamber of the housing.

18. The apparatus of claim 1 , further comprising a second heater assembly configured to cycle between at least two temperatures.

19. The apparatus of claim 18 , further comprising a plurality of amplification chambers.

20. The apparatus of claim 19 , further comprising an automated mechanism configured to, under software control, position the plurality of amplification chambers in thermal communication with the second heater assembly, wherein the second heater assembly is configured to maintain a substantially uniform temperature at the at least two temperatures in at least one amplification chamber of the plurality of amplification chambers, and wherein the second heater assembly is configured to perform independent amplification reactions in the plurality of amplification chambers when the plurality of amplification chambers is received in the apparatus.

Assignees

• Handylab Inc

Inventors

• Williams Jeff
• Wilson Kerry
• Handique Kalyan

Classifications

• B01L3/5027 Primary

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• F16K99/0061

  actuated by an expanding gas or liquid volume · CPC title

• B01L2400/0677

  phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers · CPC title

• B01L2300/1822

  using Peltier elements · CPC title

• F16K99/003

  Valves for single use only · CPC title