Micro analysis chip and fabrication method thereof

Patent metadata

Field	Value
Publication number	US-10875016-B2
Application number	US-201815899982-A
Country	US
Kind code	B2
Filing date	Feb 20, 2018
Priority date	Aug 20, 2015
Publication date	Dec 29, 2020
Grant date	Dec 29, 2020

Abstract

A micro analysis chip comprises an inlet and a fluid flow path communicating thereto. The fluid flow path comprises a first flow path, a second flow path, and a third flow path arranged continuously along a longitudinal direction of the fluid flow path. An antibody is bound on at least one peripheral surface selected from the group consisting of peripheral surfaces of the second and third flow paths. A cross-sectional area of the third flow path is constant or increased monotonically along a direction X from the second flow path toward the third flow path. A cross-sectional area of the second flow path is increased monotonically along the direction X from the one end to the other end of the second flow path. A cross-sectional area of the first flow path is larger than a cross-sectional area at the one end of the second flow path.

First claim

What is claimed is:

1. A method for fabricating a micro analysis chip, the method comprising:
(a) providing a substrate comprising a fluid flow path;
wherein:
the fluid flow path comprises a first flow path, a second flow path, and a third flow path which are arranged continuously along a longitudinal direction of the fluid flow path;
the second flow path has one end and another end;
the first flow path communicates with the second flow path through the one end of the second flow path;
the second flow path is interposed between the first flow path and the third flow path;
the second flow path communicates with the third flow path through the other end of the second flow path;
a width of the third flow path is constant or increases monotonically along a direction X from the first flow path toward the second flow path in a top view of the substrate;
a width of the second flow path increases monotonically along the direction X from the one end of the second flow path to the other end of the second flow path in the top view of the substrate; and
a width of the first flow path is larger than the width of the second flow path at the one end of the second flow path in the top view of the substrate;
(b) dropping an aqueous solution containing an antibody onto a peripheral surface of the second flow path;
wherein:
a relation LS≤L 2 +L 3 is satisfied,
where:
LS represents a length of the aqueous solution from the one end of the second flow path along the longitudinal direction of the fluid flow path;
L 2 represents a length of the second flow path along the longitudinal direction of the fluid flow path; and
L 3 represents a length of the third flow path along the longitudinal direction of the fluid flow path; and
(c) drying the aqueous solution to bind the antibody to the peripheral surface of the second flow path;
wherein:
one end of the aqueous solution is located at the one end of the second flow path;
another end of the aqueous solution moves along a direction opposite to the direction X;
the antibody is immobilized at least partially on an analysis region on the fluid flow path; and
a relation LA<LS′<LS is satisfied,
where:
LA represents a distance between the one end of the second flow path and an end of the analysis region farthest from the one end of the second flow path; and
LS′ represents the length of the aqueous solution as a result of the step (c).

2. The method according to claim 1 , wherein:
a relation LS<L 2 +L 3 is satisfied.

3. The method according to claim 1 , wherein:
the aqueous solution is also dropped onto a peripheral surface of the third flow path in the step (b).

4. The method according to claim 1 , wherein the substrate is a first substrate and the method further comprises:
(d) arranging a second substrate as a lid onto the first substrate, after the step (c).

Assignees

• Panasonic Corp
• Imec Vzw

Inventors

• Sato Shuji
• Okumura Yasuaki
• Nishiyama Yukari
• Kawamura Tatsurou
• Jones Ben
• Lagae Liesbet
• Stakenborg Tim

Classifications

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• B01L3/502707 Primary

  characterised by the manufacture of the container or its components · CPC title

• B01L3/502746

  characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves · CPC title

• B01L2400/0406

  capillary forces · CPC title

• B01L2300/0861

  Configuration of multiple channels and/or chambers in a single devices · CPC title